BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胆固醇酯酶（CHER）和胆固醇氧化酶（CHOD）经化学修饰后，与葡萄糖硫酸钠及硫化环状糊精复合物联合使用，可降低其对LDL、VLDL和乳糜微粒的酶反应性，从而仅选择性作用于HDL-胆固醇。 基于这一原理，本试剂盒采用两步法反应：第一步，使LDL、VLDL和乳糜微粒与葡萄糖硫酸钠-硫化环状糊精复合物结合，屏蔽其反应位点；第二步，利用化学修饰后的CHER和CHOD，无需预先分离其他脂蛋白，即可直接测定HDL-胆固醇。 具体反应过程如下：化学修饰的CHER催化胆固醇酯水解，生成游离胆固醇（FC）；FC在CHOD作用下被氧化，生成4-胆甾烯酮和过氧化氢（H₂O₂）。随后，H₂O₂与4-氨基安替比啉等显色剂反应，生成红色醌类化合物。该产物在546 nm波长处具有特征吸收峰，通过测定该波长下的吸光度值，即可定量计算样品中HDL-胆固醇的含量。
Cholesterol esterase (CHER) and cholesterol oxidase (CHOD), after chemical modification, are used in combination with dextran sulfate and sulfated cyclodextrin complexes. This treatment reduces their enzymatic reactivity toward LDL, VLDL, and chylomicrons, thereby allowing selective action on HDL‑cholesterol. Based on this principle, this kit employs a two‑step reaction: in the first step, LDL, VLDL, and chylomicrons are bound by the dextran sulfate–sulfated cyclodextrin complex, shielding their reactive sites; in the second step, the chemically modified CHER and CHOD are used to directly measure HDL‑cholesterol without the need for prior separation of other lipoproteins. The specific reaction process is as follows: The chemically modified CHER catalyzes the hydrolysis of cholesterol esters to generate free cholesterol (FC). FC is oxidized by CHOD to produce 4‑cholestenone and hydrogen peroxide (H₂O₂). Subsequently, H₂O₂ reacts with chromogenic reagents such as 4‑aminoantipyrine to form a red quinone compound. This product exhibits a characteristic absorbance peak at 546 nm. By measuring the absorbance at this wavelength, the HDL‑cholesterol content in the sample can be quantitatively calculated.
危险品化学品经营许可证（带存储）