BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
在生命活动的代谢过程中，机体不断产生各种自由基。羟自由基（·OH）是体内最活跃的活性氧，可介导多种生理变化。它能作用于蛋白质、核酸、脂类等生物分子，造成细胞结构和功能损伤，进而导致代谢紊乱并诱发疾病，例如引发不饱和脂肪酸的脂质过氧化反应，破坏膜结构与功能。羟自由基清除能力是评价样品抗氧化能力的重要指标之一，在抗氧化类保健品和药品研究中具有广泛应用。 本试剂盒的检测原理基于Fenton反应：H₂O₂/Fe²⁺体系产生羟自由基，同时将Fe²⁺氧化为Fe³⁺，使红色的邻二氮菲-Fe²⁺氧化为无色的邻二氮菲-Fe³⁺，导致536 nm处最大吸收峰消失。通过酶标仪测定530–540 nm范围内吸光度的变化，可计算羟自由基的含量变化，进而得出样品的羟自由基清除率或清除能力。本产品适用于植物组织、血清、血浆等样本中抗氧化能力的评估。本试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
During metabolic processes in living organisms, various free radicals are continuously generated. The hydroxyl radical (·OH) is the most reactive reactive oxygen species in vivo and can mediate a variety of physiological changes. It can act on biological macromolecules such as proteins, nucleic acids, and lipids, causing structural and functional damage to cells, subsequently leading to metabolic disorders and inducing diseases—for example, initiating lipid peroxidation of unsaturated fatty acids and disrupting membrane structure and function. The hydroxyl radical scavenging capacity is one of the important indicators for evaluating the antioxidant capacity of samples and has broad applications in the research of antioxidant health products and pharmaceuticals. The assay principle of this kit is based on the Fenton reaction: The H₂O₂/Fe²⁺ system generates hydroxyl radicals while simultaneously oxidizing Fe²⁺ to Fe³⁺, converting the red phenanthroline‑Fe²⁺ complex to the colorless phenanthroline‑Fe³⁺ complex, resulting in the disappearance of the maximum absorption peak at 536 nm. By measuring the change in absorbance within the range of 530–540 nm using a microplate reader, the change in hydroxyl radical content can be calculated, thereby yielding the hydroxyl radical scavenging rate or scavenging capacity of the sample. This product is suitable for evaluating the antioxidant capacity of samples such as plant tissues, serum, and plasma. This kit is intended for research use only and is not suitable for clinical diagnosis or other applications.
危险品化学品经营许可证（带存储）