计算溶液所需的质量、体积或浓度。
高糖高脂细胞添加剂试剂盒
有货
为什么选择此级别
BioReagent,无菌过滤,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品有效成分为 10 mM 棕榈酸钠与 1 M 葡萄糖，经 0.22 μm 过滤除菌，无菌溶液，可直接加入完全培养基。试剂盒包含高脂、高糖配套溶剂对照，可排除胶体与晶体渗透压干扰。高脂组分呈淡黄色清亮液体，葡萄糖组分无色透明；溶剂不含 NaOH 与有机溶剂，细胞毒性低，适配多种细胞，适合构建细胞脂毒性模型，用于内质网应激、氧化应激、线粒体损伤、细胞凋亡相关研究。
产品组成
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使用说明
1. 2~8℃过夜或室温解冻，如不澄清，10 mM棕榈酸钠/高脂对照可短暂水浴（≤50℃，≤3min），1 M葡萄糖/高糖对照可50℃水浴涡旋至完全溶解。改为 10mM棕榈酸钠高脂细胞添加剂/高脂对照 可短暂水浴（≤50℃，≤3min）， 1M葡萄糖细胞添加剂/高糖对照(甘露醇1M) 可50℃水浴涡旋至完全溶解。
2. 针对不同细胞种类及实验目的，棕榈酸钠、葡萄糖所用浓度和比例不同，处理时间也不同，请根据实验需求进行预实验。
3. 对于首次使用本产品，建议采用浓度梯度处理细胞，摸索针对特定细胞、达到特定效果的适宜浓度。
4. 待细胞生长至 80-100%汇合度时，换成新鲜培养基（含血清），按适当浓度滴加棕榈酸钠/葡萄糖，对照组加入等量体积（棕榈酸钠+葡萄糖体积）对照，轻柔混匀后将细胞放入细胞培养箱中继续培养。改为按适当浓度滴加 10mM棕榈酸钠高脂细胞添加剂/1M葡萄糖细胞添加剂，对照组加入等量体积高脂对照/高糖对照(甘露醇1M) ，轻柔混匀后将细胞放入细胞培养箱中继续培养。
5. 加入本品24~48小时后，进行下一步分析。
注意事项
1. 收到产品后可分装，于-20℃长期保存；可在2~8℃短期保存。
2. 由于溶剂BSA影响吸光度，在使用CCK-8检测细胞毒性时，在弃掉高脂细胞添加剂与培养基混液后，需要用PBS清洗细胞2~3次，再加入CCK-8工作液。
This product contains active ingredients of 10 mM sodium palmitate and 1 M glucose. It is a sterile solution sterilized by 0.22 μm filtration and can be directly added to complete medium. The kit is supplied with matched high‑fat and high‑glucose solvent controls to eliminate interference from colloidal and crystalloid osmotic pressure. The high‑fat components are pale‑yellow clear liquids, while the glucose components are colorless and transparent. The solvent system contains neither NaOH nor organic solvents and exhibits low cytotoxicity. Compatible with multiple cell types, this product is suitable for establishing cellular lipotoxicity models to conduct research on endoplasmic reticulum stress, oxidative stress, mitochondrial damage and cell apoptosis.
Product Components
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Instructions for Use
1.Thaw overnight at 2–8 °C or at room temperature. If turbidity appears, 10 mM sodium palmitate / high-fat control may undergo short-term water bath treatment (≤50 °C, ≤3 min); 1 M glucose / high-glucose control can be incubated in a 50 °C water bath and vortexed until fully dissolved.改为 10 mM Sodium Palmitate High-Fat Cell Supplement / High-Fat Control may undergo short-term water bath treatment (≤50 °C, ≤3 min); 1 M Glucose High-Glucose Cell Supplement / High-Glucose Control (1 M Mannitol) can be incubated in a 50 °C water bath and vortexed until fully dissolved.
2.The working concentration, ratio and treatment duration of sodium palmitate and glucose vary depending on cell type and experimental objectives. Preliminary tests should be performed according to experimental requirements.
3.For first-time use, it is recommended to treat cells with a concentration gradient to determine the optimal concentration for specific cells and expected phenotypes.
4.When cells reach 80–100% confluence, replace with fresh serum-containing medium. Add sodium palmitate or glucose at an appropriate concentration, and add an equal volume of control solution (matching the total volume of sodium palmitate plus glucose) to the control group. Mix gently and incubate cells in a cell incubator.改为 Add 10 mM Sodium Palmitate High-Fat Cell Supplement / 1 M Glucose High-Glucose Cell Supplement at an appropriate concentration, and add an equal volume of control solution High-Fat Control / High-Glucose Control (1 M Mannitol) to the control group.
5.Proceed with subsequent analysis 24–48 hours after treatment.
Precautions
1.Aliquot the product upon receipt for long-term storage at -20 °C; short-term storage is acceptable at 2–8 °C.
2.BSA in the solvent affects absorbance readings. When assessing cytotoxicity via CCK-8 assay, remove the mixture of high-fat cell supplement and medium, rinse cells 2–3 times with PBS, and then add CCK-8 working solution.
规格
规格或纯度
无菌过滤, BioReagent, 用于细胞培养, 棕榈酸钠 10mM; 葡萄糖 1M
英文名称
High‑Glucose & High‑Fat Cell Additive Kit
应用
Cell Culture
储存与运输
储存条件
-20°C储存
运输条件
超低温运输
稳定性与储存
Store at -20°C long term (24 months).
组分与储存
| 组分货号 | 组分名称 | 物理外观 | 4×5mL | 储存 |
| H1527955A | 10mM棕榈酸钠高脂细胞添加剂 | 液体 | 5 mL | -20°C |
| H1527955B | 高脂对照 | 液体 | 5 mL | -20°C |
| H1527955C | 1M葡萄糖细胞添加剂 | 液体 | 5 mL | -20°C |
| H1527955D | 高糖对照(甘露醇1M) | 液体 | 5 mL | -20°C |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
溶液计算器
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