HEPES缓冲液 (20mM, pH 7.4, 无菌, 无酶)

Name: HEPES缓冲液 (20mM, pH 7.4, 无菌, 无酶)
SKU: H1529368
Availability: InStock

CAS: 7365-45-9 货号: H1529368 分子式: C₈H₁₈N₂O₄S 分子量: 238.30 EC号: 230-907-9

592篇引用文献 COA

有货

级别和纯度: 无菌过滤 ? BioReagent ? 用于细胞培养 ? 20mM in ddH₂O

储存条件

2-8°C储存

运输条件

低温运输

应用

Cell Culture

规格

库存

价格

数量

100ml

H1529368-100ml

期货

¥199.90

500ml

H1529368-500ml

期货

¥579.90

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概述规格名称和识别符技术文档高级数据产品属性质检证书（CoA,COO,BSE/TSE 和分析图谱) 化学和物理性质安全和危险性（GHS）此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

BioReagent,无菌过滤,用于细胞培养级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 592 篇同行评审文献引用。

概述

HEPES (free acid)即4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid，中文名为4-羟乙基哌嗪乙磺酸(游离酸)、4-(2-羟乙基)哌嗪-1-乙磺酸(游离酸)或N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)(游离酸)。HEPES是一种常用的两性离子缓冲试剂，有效缓冲范围为pH6.8-8.2，pKa(25℃)=7.5。

HEPES缓冲液 (20mM, pH 7.4, 无菌, 无酶) 常用于细胞培养，一般工作浓度为10-25mM。相比于低pKa的碳酸氢盐缓冲液，HEPES的pKa为7.5，在pH7.2-7.6范围内具有良好的缓冲能力，适合需要精确pH条件的细胞培养，同时在开放式培养或细胞长时间离开CO2培养箱时 (如细胞观察)，HEPES也能提供良好的缓冲能力。

HEPES是很多细胞培养体系缓冲液的优选，因为HEPES对细胞膜无通透性，对各种生化反应影响很小，化学和酶学稳定，并且具有非常低的可见光和UV光吸收。

HEPES在浓度为10-25mM时对细胞无毒性，但当浓度超过40mM后可能对某些细胞产生毒性，影响细胞生长。

注意事项：

1、本产品经0.1μm过滤除菌；

2、使用本产品时应注意无菌操作，避免污染；

3、使用后请及时避光保存，有研究报道培养液中的HEPES在可见光下暴露3h以上将产生对细胞有毒性的过氧化氢；

4、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。

HEPES (free‑acid), full chemical name: 4‑(2‑Hydroxyethyl)piperazine‑1‑ethanesulfonic acid. Its Chinese aliases include 4‑hydroxyethylpiperazine ethanesulfonic acid (free acid), 4‑(2‑hydroxyethyl)piperazine‑1‑ethanesulfonic acid (free acid), or N‑(2‑hydroxyethyl)piperazine‑N′‑(2‑ethanesulfonic acid) (free acid). HEPES is a widely‑used zwitterionic buffering reagent with an effective buffering range of pH 6.8‑8.2 and a pKa value of 7.5 at 25 °C.

HEPES Buffer Solution (20mM, pH 7.4, Sterile, Enzyme‑free) is commonly applied in cell culture, with a typical working concentration of 10‑25 mM. Compared with bicarbonate‑based buffers that have a lower pKa, HEPES exhibits excellent buffering capacity in the pH range of 7.2‑7.6 owing to its pKa of 7.5. It is suitable for cell‑culture experiments requiring precise pH control. Meanwhile, HEPES can maintain stable pH buffering performance under open‑culture conditions or when cells are removed from the CO₂ incubator for an extended period (e.g., during cell observation).

HEPES is a preferred buffer component for numerous cell‑culture systems. It is membrane‑impermeable, barely interferes with diverse biochemical reactions, features high chemical and enzymatic stability, and shows very low absorbance in the visible and ultraviolet spectral regions.

HEPES is non‑toxic to most cell lines at concentrations between 10‑25 mM; nevertheless, concentrations above 40 mM may be cytotoxic to certain cell types and inhibit cell proliferation.

Precautions

1. This product is sterilized by filtration through a 0.1 μm filter membrane.

2. Aseptic operation should be performed during use to avoid contamination.

3. Store in dark place promptly after opening. Studies have reported that HEPES in culture medium can produce cytotoxic hydrogen peroxide upon exposure to visible light for more than 3 hours.

4. This product is intended for scientific research or further‑research purposes only. It shall not be used for diagnosis or treatment.