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EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 55 篇同行评审文献引用。
透明质酸酶广泛存在于自然界。在人体中,它存在于不少器官和体液中。透明质酸酶见于病毒、噬菌体、真菌和细菌的分泌物。它还存在于线虫和蚂蟥的分泌物中。蛇和蝎子的毒液中会产生透明质酸酶。蜜蜂、蜘蛛、黄蜂、大黄蜂、毛虫、鱼类和蜥蜴也会分泌透明质酸酶。 透明质酸酶属于内切糖苷酶,通过配合β-葡糖醛酸酶和β-N-乙酰氨基葡萄糖苷酶等外切糖苷酶完成其内切功能。透明质酸酶调节透明质酸的周转代谢。 透明质酸裂解酶在β-D-GlcNAc-(1→4)-β-D-GlcA键上切割透明质酸,得到3-(4-脱氧β-D-葡萄糖-4-烯基糖基)-N-乙酰基-D-葡糖胺四糖和六糖。与其他透明质酸酶不同,该酶对透明质酸具有特异性,并且对软骨素和硫酸软骨素无活性。
Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards. Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover. Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.