K2202734计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Description：
HRP Maleimide Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated HRP contains 1–3 maleimide groups for each HRP molecule.
Product Information：
Volume/Quantity : 0.5 mg x 3 vial
Conjugation : Peroxidase from Horseradish (HRP) was conjugated to Sulfo-SMCC (Product M123456).
Product Form : Horseradish Peroxidase Maleimide Conjugate. Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl, 40 mM sucrose, pH 6.6.
Preservative : None
Protocol：
Firstly return the vial to room temperature.
Reconstitute the contents of the vial with 100μL of buffer or water to obtain a 5 mg/mL solution of HRP Maleimide.
Note: Do not vortex or heat the HRP Maleimide.
Dissolve the sulfhydryl compound to be conjugated at 2-5 mg/ml in the buffer.
Note: We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 10mM EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. Use of a 4:1 (HRP Maleimide:antibody) molar ratio in the conjugation reaction usually results in high-activity conjugates. This vial contains 0.5 mg of HRP Maleimide Conjugate and can effectively modify 0.5 mg of immunoglobulin.
Immediately mix the thiol-containing molecule and HRP Maleimide solutions and allow them to react for 2 hours at room temperature. To increase HRP incorporation, extend reaction time up to 12 hours.
If desired, desalt, dialyze or purify the conjugate.
Storage：
Store at −20 °C to −80 °C. Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
规格
产品名称
辣根过氧化物酶马来酰亚胺结合物 (0.5mg×3)
别名
辣根过氧化物酶马来酰亚胺活化物 (0.5mg×3) | HRP马来酰亚胺 (0.5mg×3)
英文别名
HRP Maleimide (0.5mg×3) | Horseradish Peroxidase Maleimide Activated (0.5mg×3)为：HRP Maleimide (0.5mg×3) | Horseradish Peroxidase Maleimide Activated (0.5mg×3)
CAS编号和信息
H405810
分子类型
酶
储存与运输
物理形态
固体
储存条件
-20°C储存
运输条件
超低温运输
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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