计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
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文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
血红蛋白 (Hemoglobin ，Hb 或 HGB) 是高等生物体内负责运载氧的一种蛋白质，是能使血液呈红色的蛋白。血红蛋白由四条链组成,两条α链和两条β链,每一条链有一个包含一个铁原子的环状血红素。Hb 在氧含量高的区域,容易与氧结合；在氧含量低的区域,又容易与氧分离。血红蛋白的这一特性，使红细胞具有运输氧的功能。
BIOISCO 血红蛋白检测试剂盒 (微板法 )( Hemoglobin Colorimetric Assay Kit) 其检测原理是血红蛋白中亚铁血红素有类似过氧化物酶的作用，可在氧化剂的作用下催化显色底物，在酸性条件下产物呈红色，吸收峰，产物红色越深，说明 Hb 含量越高，反之则越低,通过酶标仪测定处吸光度，据此通过比色分析就可以计算出血清血红蛋白含量水平。该试剂盒主要用于检测溶血血清样本，亦可用于检测细胞或组织的裂解液或匀浆液等中血红蛋白含量。该试剂盒仅用于科研领域,不宜用于临床诊断或其他用途。
存储条件：
按要求保存，半年有效。
自备材料：
生理盐水、96 孔板、水浴锅或恒温箱、酶标仪
组分：
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注意事项：
1、Hb(1mg/ml)和标准品工作液 (13.2 μg/ml)均应 -20 ℃保存，同时应避免反复冻融。
2、如果没有酶标仪，也可以使用普通的分光光度计测定，但应考虑根据比色杯的最小检测体积，尽量采
用小体积的比色杯。
3、1 支显色工作液配制后应尽快用完，因此请注意适当多准备一些样品一起检测。
操作步骤（仅供参考）：
1、 配制检测工作液：
①配制标准品工作液：取出标准品恢复至室温，准确取 10μl，加入注射用水 90μl，即为标准品工作液 。
（临用现配）
①配制试剂二工作液：取出试剂二恢复至室温，将其按照 1：29 的比例进行稀释，即为试剂二工作液 。
（根据需要用量来配制，临用现配）
2 、 准备样品：
①溶血标本血清：溶血血清按照常规方法制备后，-20 ℃冻存，使用时用生理盐水稀释。
②细胞或组织样品：取恰当的细胞或组织裂解液，如果有必要需进行适当匀浆，低
速离心取上清，-20 ℃冻存。
3、 加样体系：按照下表设置 96 孔板空白孔、标准孔、测定孔，溶液应按照顺序依次
加入，并注意避免产生气泡。如果样品中的血红蛋白含量过高，可以减少样品用量或适
当稀释后再进行测定。样品的检测最好能设置平行孔，尤其是标准品应作三复孔。
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4、 酶标仪检测：435nm 处吸光度即 A435 ，如果无法检测，亦可检测吸光度，一般应数小时内检测完毕。
计算结果：
血清血红蛋白 (mg/L)= 待测样品吸光度 / 标准品吸光度× 13.2 ×100(mg/L)
Hemoglobin (Hb or HGB) is a protein responsible for carrying oxygen in higher organisms and is the protein that makes blood appear red. Hemoglobin is composed of four chains, two alpha chains and two beta chains, each with a cyclic heme containing one iron atom. Hb is easily bound to oxygen in areas with high oxygen content; In areas with low oxygen content, it is easy to separate from oxygen. The characteristic of hemoglobin enables red blood cells to transport oxygen.
The detection principle of BIOISCO Hemoglobin Colorimetric Assay Kit is that heme in hemoglobin has a peroxidase like effect, which can catalyze the chromogenic substrate under the action of oxidants. Under acidic conditions, the product appears red with an absorption peak. The darker the red color of the product, the higher the Hb content, otherwise it is lower. The absorbance at the site is measured by an enzyme-linked immunosorbent assay (ELISA) reader, and the hemoglobin content level in the serum can be calculated through colorimetric analysis. This kit is mainly used to detect hemolytic serum samples, and can also be used to detect hemoglobin content in cell or tissue lysates or homogenates. This kit is only used in the field of scientific research and should not be used for clinical diagnosis or other purposes.
Storage conditions:
Save as required, valid for six months.
Self provided materials:
Physiological saline, 96 well plate, water bath or constant temperature box, enzyme-linked immunosorbent assay (ELISA) reader
Components:
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Matters needing attention:
1. Hb (1mg/ml) and standard working solution (13.2 μ g/ml) should be stored at -20 ℃, and repeated freezing and thawing should be avoided.
2. If there is no enzyme-linked immunosorbent assay (ELISA) reader, a regular spectrophotometer can also be used for measurement, but consideration should be given to using the minimum detection volume of the colorimetric cup as much as possible
Use a small-sized colorimetric cup.
3. After preparing one color developing working solution, it should be used up as soon as possible, so please prepare more samples for testing.
Operation steps (for reference only):
1. Preparation of testing working fluid:
① Preparation of standard working solution: Take out the standard and restore it to room temperature. Accurately take 10 μ l and add 90 μ l of injection water to obtain the standard working solution.
(Ready to use and equipped)
① Preparation of reagent 2 working solution: Take out reagent 2 and restore it to room temperature. Dilute it in a ratio of 1:29 to obtain reagent 2 working solution.
(Prepare according to the required dosage, ready for immediate use)
2. Prepare samples:
① Hemolytic specimen serum: Hemolytic serum is prepared according to conventional methods, frozen at -20 ℃, and diluted with physiological saline when used.
② Cell or tissue samples: Take appropriate cell or tissue lysate, and if necessary, perform appropriate homogenization
Centrifuge the supernatant and freeze it at -20 ℃.
3. Sample addition system: Set the blank well, standard well, and measuring well of the 96 well plate according to the following table, and the solution should be prepared in sequence
Add and be careful to avoid creating bubbles. If the hemoglobin content in the sample is too high, the sample dosage can be reduced or adjusted appropriately
Measure after dilution. It is best to set parallel holes for sample testing, especially for standard samples, which should have three replicates.
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4. Enzyme linked immunosorbent assay (ELISA) detection: The absorbance at 435nm is A435. If it cannot be detected, the absorbance can also be detected, which should generally be completed within a few hours.
Calculation result:
Serum hemoglobin (mg/L)=absorbance of the test sample/absorbance of the standard sample × 13.2 × 100 (mg/L)
规格
英文名称
Hemoglobin Assay Kit
敏感性
对光敏感
法律信息
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
储存与运输
储存条件
避光,-20°C储存
运输条件
超低温运输
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高级数据
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|---|---|---|
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溶液计算器
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