计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Hoechst 33258 analog is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
In Vitro
General Protocol Preparation of Hoechst working solution 1.1 Preparation of the stock solution Dissolve 10 mg of in 5 mL ddH 2 O Note: It is recommended to store the stock solution at 4°C or -20°C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Hoechst working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working solution according to the actual situation. 1.Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. Storage 4°C, 1 year. Protect from light Precautions 1. Please adjust the concentration of Hoechst working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells . MCE has not independently confirmed the accuracy of these methods. They are for reference only.
规格
规格或纯度
10mM in DMSO
英文名称
Hoechst 33258 analog
生化机理
Hoechst 33258 类似物是 Hoechst 系列中的一种标记染料。Hoechst 是一种活的核标记染料。Hoechst 能与 DNA 双链的沟槽结合，这种双链往往是富含 A/- T 的 DNA 链。虽然它与所有核酸都能结合，但富含 A/- T 的双股 DNA 链与所有核酸都能结合。
储存条件
避光,-80℃储存
运输条件
特低温运输
名称和识别符
分子类型
小分子
Isomeric SMILES
CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC(=CC=C6)OCCCC(=O)O
关联CAS
分子量
510.59
技术文档
高级数据
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