透明质酸酶(只降解透明质酸)

CAS: 37326-33-3 货号: H766309 EC号: 253-464-3
有货
级别和纯度: 重组 ? 重组(Recombinant)—— 通过重组表达生产,序列确定、批次稳定。适用于来源明确、无动物源的可重现蛋白。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 expressed in E.coli;≥1000 U/mg enzyme powder; ≥2000 U/mg protein
表达系统
E.coli & Pichia pastoris
生物活性
≥1000 U/mg enzyme powder; ≥2000 U/mg protein
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规格
库存
价格
数量
100U
H766309-100U
现货 Stock Image
¥699.90
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为什么选择此级别

EnzymoPure™,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 20 篇同行评审文献引用。

概述

微生物来源的透明质酸(HA)降解酶属于多糖裂解酶,因此通常被称为HA裂解酶(HAase)(EC 4.2.2.1),该类酶通过β-消除反应断裂HA糖链中N-乙酰葡萄糖胺(GlcNAc)和葡萄糖醛酸(GlcUA)之间的β-1,4糖苷键,并在糖醛酸的C4和C5位上产生一个不饱和双键,该双键在232 nm有特征吸收,可以方便地用于寡糖产物分析检测。HAase已在多种细菌中被发现,如Proteus, Bacteroides fragilis, Streptococcus, Staphylococcus, Peptostreptococcus, Propionibacterium, Streptomyces, Clostridium, and Vibrio等。 许多HAase同时具有软骨素/硫酸软骨素降解活性,这类HAase常被归类到硫酸软骨素酶CSase AC。许多HAases在降解底物时起初表现为内切酶特点,对HA/CS多糖链进行随机结合并切割,随后则表现为外切酶特性,因此在底物降解过程中往往观测到不饱和二糖产物快速积累,但其它高聚合度寡糖占比则始终很低。 在我们的产品中,为了避免混淆,我们将同时具有HA和CS降解活性的HAases归入CSase AC,可以根据客户的需要提供各种具有不同比酶活和底物结构选择性的HAases。在此,HAase指的是来自于Streptomyces hyalurolyticus等的HA专一性降解酶,可以作为生物样品中HA分析检测和HA样品纯度检测的专一性工具酶。

Microbial derived hyaluronic acid (HA) degrading enzymes belong to polysaccharide lyases and are commonly referred to as HA lyases (EC 4.2.2.1). These enzymes break the β -1,4-glycosidic bond between N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcUA) in the HA sugar chain through a β - elimination reaction, and produce an unsaturated double bond at the C4 and C5 positions of the glucuronic acid. This double bond has characteristic absorption at 232 nm and can be conveniently used for oligosaccharide product analysis and detection. HAase has been found in various bacteria, such as Proteus, Bacteroides fragilis, Streptococcus, Staphylococcus, Peptostreptococcus, Propionibacterium, Streptomyces, Clostridium, and Vibrio Wait. Many HAases have both chondroitin sulfate/chondroitin sulfate degradation activity, and these HAases are often classified as chondroitin sulfate CSase AC. Many HAases initially exhibit endonuclease characteristics when degrading substrates, randomly binding and cleaving HA/CS polysaccharide chains, followed by exonuclease characteristics. Therefore, rapid accumulation of unsaturated disaccharide products is often observed during substrate degradation, while the proportion of other high degree of polymerization oligosaccharides remains low. In our product, to avoid confusion, we classify HAases with both HA and CS degradation activities as CSase AC. We can provide various HAases with different specific enzyme activities and substrate structure selectivity according to customer needs. Here, HAase refers to HA specific degrading enzymes from Streptomyces hyalurolyticus and others, which can be used as specific tool enzymes for HA analysis and purity detection in biological samples.

规格

产品名称
透明质酸酶(只降解透明质酸)
别名
透明质酸裂解酶(只降解透明质酸) | 透明质酸裂解酶(只降解透明质酸) | HA裂解酶
英文别名
hyaluronate lyase | hyaluronidase | mucinase | spreading factor | HAase
规格或纯度
重组, EnzymoPure™, expressed in E.coli;≥1000 U/mg enzyme powder; ≥2000 U/mg protein
生物活性
≥1000 U/mg enzyme powder; ≥2000 U/mg protein
表达系统
E.coli & Pichia pastoris
来源
重组表达
CAS编号和信息
37326-33-3
酶学委员会编号
EC 4.2.2.1
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
expressed in E.coli;≥1000 U/mg enzyme powder; ≥2000 U/mg protein
储存条件
-80℃储存
运输条件
特低温运输
单位定义
在 37 ºC、pH 6.0 条件下,一单位每分钟可降解 0.75 μg 多糖透明质酸(通过比浊吸光度测量(λ: 600nm)。

技术文档

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🔬 规格说明书

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高级数据

三维结构
交互式化学结构模型





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找到2个结果

批号(Lot Number) 证书类型 货号
E2521372 分析证书 H766309
E2521435 分析证书 H766309
技术文档和文章
此产品的引用文献
引用文献
1. Yidan Chen, Tiantian Feng, Xiaohong Zhu, Yuting Tang, Yao Xiao, Xiuhua Zhang, Sheng-Fu Wang, Dong Wang, Wei Wen, Jichao Liang, Huayu Xiong.  (2024)  Ambient Synthesis of Porphyrin-Based Fe-Covalent Organic Frameworks for Efficient Infected Skin Wound Healing.  BIOMACROMOLECULES,  [PMID:38720431] [10.1021/acs.biomac.4c00261]
2. Mengyao Qi, Yi Ke, Yifei Li, Peng Li, Huakuo Zhou, Xia Zhang, Jieshi Chen, Jun Meng.  (2024)  Au@Pt nanozyme-based smart hydrogel for visual detection of hyaluronic acid.  SENSORS AND ACTUATORS B-CHEMICAL,  [10.1016/j.snb.2024.137144]
3. Tao Meng, Zhengyu Zhao, Mengqiang Song, Rui Wang, Xu Xu, Min Yang, Weihua Li, Shiyong Song.  (2025)  Carboxybetaine modified chitosan as viscosupplementation for osteoarthritis therapy.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  [PMID:40090277] [10.1016/j.ijbiomac.2025.142155]
4. Yu Pang, Jie Lv, Chengcai He, Chengda Ju, Yulong Lin, Cong Zhang, Meng Li.  (2024)  Covalent organic frameworks-derived carbon nanospheres based nanoplatform for tumor specific synergistic therapy via oxidative stress amplification and calcium overload.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,  [PMID:38330663] [10.1016/j.jcis.2024.01.217]
5. Siying Huang, Simeng Xiao, Xuehao Li, Ranran Tao, Zhangwei Yang, Ziwei Gao, Junjie Hu, Yan Meng, Guohua Zheng, Xinyan Chen.  (2024)  Development of Dual-Targeted Mixed Micelles Loaded with Celastrol and Evaluation on Triple-Negative Breast Cancer Therapy.  Pharmaceutics,  16  (9): (1174).  [PMID:39339211] [10.3390/pharmaceutics16091174]
6. Shiyi Yao, Hui Yuan, Luqi Yang, Yin Zhang, Hanyu Wang, Renxuan Li, Tingjun Ye, Wenguo Cui, Lei Wang.  (2025)  Downregulation of the PI3K/AKT/mTOR/MMP-13 pathway for promoting interface healing via lubricating microspheres.  Acta Biomaterialia,  [PMID:39761787] [10.1016/j.actbio.2025.01.001]
7. Jing Zhang, Yunfeng Zeng, Yongyuan Heng, Yu Shen, Ximeng Sun, Yijia Wang, Haiping Zheng, Ming Zeng, Ziyi Yu.  (2024)  Enzyme-Assisted Activation Technique for Producing Versatile Hydrogel Microparticle Scaffolds with High Surface Chemical Reactivity.  ADVANCED FUNCTIONAL MATERIALS,  [10.1002/adfm.202400858]
8. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li.  (2024)  Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound.  ACS Nano,  [PMID:38949962] [10.1021/acsnano.4c02921]
9. Huiping Yang, Yinning Lin, Qian Mo, Zhaoquan Li, Fan Yang, Xinchun Li.  (2024)  Monitoring Enzymatic Reaction Kinetics and Activity Assays in Confined Nanospace.  ANALYTICAL CHEMISTRY,  [PMID:39024010] [10.1021/acs.analchem.4c01901]
10. Shuo Yu, Lu Zhang, Yanshen Yang, Meijuan Wang, Tingting Liu, Wenwen Ji, Yang Liu, Hao Lv, Yang Zhao, Xi Chen, Tinghua Hu.  (2024)  Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model.  ACS Pharmacology & Translational Science,  [PMID:38633596] [10.1021/acsptsci.3c00304]
11. Xin Jin, Chengxiong Wei, Kai Li, Peinan Yin, Chengwei Wu, Wei Zhang.  (2024)  Polyphenol-mediated hyaluronic acid/tannic acid hydrogel with short gelation time and high adhesion strength for accelerating wound healing.  CARBOHYDRATE POLYMERS,  [PMID:39048222] [10.1016/j.carbpol.2024.122372]
12. Dandan Wei, Guanhua Jiao, Yinghua Tao, Liuxin Yang, Tao Liu, Fengya Jing, Tianzhu Zhang.  (2025)  Polypropylene Mesh Coated with Dual Cross-Linked Hyaluronic Acid/Polyvinyl Alcohol Composite Hydrogel with Antiadhesion and Angiogenesis Properties for Abdominal Wall Repair.  Advanced Materials Technologies,  [10.1002/admt.202401786]
13. Dandan Wei, Yulin Huang, Min Liang, Liuxin Yang, Guanhua Jiao, Yinghua Tao, Li Xu, Tianzhu Zhang, Zhenling Ji.  (2024)  Polypropylene mesh coated with hyaluronic acid/polyvinyl alcohol composite hydrogel for preventing bowel adhesion.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,  [PMID:38705326] [10.1016/j.ijbiomac.2024.132061]
14. Chao-fan He, Tian-hong Qiao, Xu-chao Ren, Mingjun Xie, Qing Gao, Chao-qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He.  (2025)  Printability in Multi-material Projection-Based 3-Dimensional Bioprinting.  Research,  [PMID:40041038] [10.34133/research.0613]
15. Zhu Dan, Zhong Hanyan, Shi Jingzhan, Liu Qiang, Wang Yiping.  (2025)  Ratiometric surface-enhanced Raman scattering quantification of extracellular matrix metalloproteinase-2 activity for tumor diagnosis.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  [PMID:39966175] [10.1007/s00216-025-05792-5]
16. Yingying Chen, Dan Zhu, Hanyan Zhong, Zhixing Gan, Shenfei Zong, Zhuyuan Wang, Yiping Cui, Yiping Wang.  (2024)  Ultrasensitive Detection of Matrix Metalloproteinase 2 Activity Using a Ratiometric Surface-Enhanced Raman Scattering Nanosensor with a Core–Satellite Structure.  ACS Applied Materials & Interfaces,  [PMID:38204415] [10.1021/acsami.3c15344]
17. Shangting Du, Lingyu Li, Junhao Kou, Tianyi Zhu, Zhenyi Song, Yonghua Zhan, Daocheng Wu, Wenhua Zhan.  (2025)  Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy.  International Journal of Nanomedicine,  [PMID:40692537] [10.2147/IJN.S530041]
18. Jiancong Ni, Xiaochun Xu, Bifang Yang, Liyang Liu, Weiqiang Yang, Xiaoping Chen, Guiping Chen, Qingxiang Wang, Zhenyu Lin.  (2025)  Indicator-Free Detection of Hyaluronidase Based on a Conductivity-Regulated Bipolar Electrochemiluminescence Platform.  ANALYTICAL CHEMISTRY,  [PMID:40827572] [10.1021/acs.analchem.5c03409]
19. Haojie Qiu, Jingyan Zhu, Kaikai Zhang, Xinnan Wang, Yaoting Chu, Feng Zhang, Xuefeng Yang.  (2025)  Dual Drug-Loaded Hyaluronate-Based Self-Adaptable Hydrogel for Diabetic Infected Wound Healing.  BIOMACROMOLECULES,  [PMID:40845349] [10.1021/acs.biomac.5c00712]
20. Wenjun Xue, Xiaoyu Kuang, Xuanzhen Meng, Boyu Sun, Zijun Zhao, Guizhao Liang.  (2025)  Identification and inhibition mechanism of novel collagen-derived hyaluronidase inhibitory peptides.  Food Bioscience,  [10.1016/j.fbio.2025.106263]
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