计算溶液所需的质量、体积或浓度。
改良Page髓鞘染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
髓鞘 (Myelin Sheath)是包裹在神经细胞轴突外面的一层膜，即髓鞘由髓鞘细胞和细胞膜组成，是神经膜细胞的质膜沿着轴索的轴心螺旋缠绕形成的多层脂双层结构, 髓鞘上有郎飞氏结，可使神经冲动跳跃传递，髓鞘染色在病理诊断中有一定意义，髓鞘的病理变化分为早期、中期和晚期。在早期着色较深；病变中期阶段的髓鞘变性形成脂滴，可用脂质染色加以显示，后期彻底溃变并被吞噬细胞清除，故不再有髓鞘的阳性结果。
很多疾病都可以引起髓鞘的变化，改良Page髓鞘染色液又称砂罗铬花青髓鞘染色液，可以显示病理情况下髓鞘是否完整、变性、坏死程度及修复情况，对神经组织的病理诊断和研究均有意义, 髓鞘呈蓝色，脱髓鞘纤维不着色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
蒸馏水、梯度乙醇、二甲苯或脱蜡透明液、中性树胶
操作步骤(仅供参考)：
1、组织固定于的Page甲醛固定液中，固定时间应大于3天以上。
2、常规脱水包埋，切片厚度5μm，切片二甲苯或脱蜡透明液脱蜡至蒸馏水。
3、用改良Page染色液滴染15-25min。
4、倾去染色液，流水冲洗1min。
5、取适量的Page分化液，按Page分化液: 蒸馏水=1: 1的比例配制Page分化工作液；切片直接用Page分化工作液分化20-180s，该分化过程需在显微镜下观察控制分化程度。
6、自来水冲洗10min。
7、入Page桃红染色液复染3-6s(见注意事项3)，自来水稍洗。
8、常规梯度乙醇脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
髓鞘、细胞核: 深蓝色
红细胞: 绿色
细胞胞质、胶原纤维、肌纤维: 桃红色
注意事项：
1、分化这一步很关键，应严格控制分化时间，可在镜下观察分化程度，可先分化几秒，水洗后显微镜下观察，直至胶原纤维和肌纤维接近于无色或淡灰色，髓鞘呈清晰的蓝色为止。
2、如果该分化过程不易控制，可用蒸馏水把Page分化液稀释4-5倍后再行分化。
3、切片不宜太厚，应控制在5-6μm以内，否则易出现脱片或过染等现象。
4、新的Page桃红染色液染色时间较短，而保存较久的Page桃红染色液染色力会下降，可稍微温热后染色数分钟。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Myelin Sheath is a membrane wrapping around the axons of nerve cells, i.e., it is composed of myelinating cells and cell membranes, forming a multi-layered lipid bilayer structure by the plasma membrane of neurolemmocytes spirally winding along the axis of axons. The myelin sheath has nodes of Ranvier, which enable the saltatory conduction of nerve impulses. Myelin staining is of certain significance in pathological diagnosis. Pathological changes of the myelin sheath are divided into early, intermediate and late stages. In the early stage, the staining is relatively deep; in the intermediate stage of lesions, myelin degeneration forms lipid droplets, which can be displayed by lipid staining; in the late stage, the myelin sheath completely degenerates and is cleared by phagocytes, so there will be no positive result of myelin sheath.
Many diseases can cause changes in the myelin sheath. Modified Page Myelin Staining Solution, also known as Gallocyanin Myelin Staining Solution, can display the integrity, degeneration, necrosis degree and repair status of the myelin sheath under pathological conditions, which is of significance for the pathological diagnosis and research of nerve tissues. The myelin sheath appears blue, while demyelinated fibers are unstained. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by User
Distilled water, gradient ethanol, xylene or dewaxing clearing solution, neutral mounting medium
Procedure (for reference only)
1. Fix the tissue in Page Formaldehyde Fixative for more than 3 days.
2. Perform routine dehydration and embedding, slice into sections with a thickness of 5 μm, then dewax the sections to distilled water using xylene or dewaxing clearing solution.
3. Stain the sections with Modified Page Staining Solution for 15-25 minutes.
4. Pour off the staining solution and rinse the sections with running water for 1 minute.
5. Take an appropriate amount of Page Differentiation Solution, prepare Page Differentiation Working Solution at a ratio of Page Differentiation Solution: distilled water=1:1; differentiate the sections directly with the working solution for 20-180 seconds. The differentiation process should be observed under a microscope to control the differentiation degree.
6. Rinse the sections with tap water for 10 minutes.
7. Counterstain the sections with Page Fuchsine Staining Solution for 3-6 seconds (see Precaution 3), then rinse briefly with tap water.
8. Perform routine dehydration with gradient ethanol, clear the sections with xylene or dewaxing clearing solution, and mount with neutral mounting medium.
Staining Results
Myelin sheath, cell nuclei: Dark blue
Red blood cells: Green
Cytoplasm, collagen fibers, muscle fibers: Fuchsine
Precautions
1. Differentiation is a critical step, and the differentiation time should be strictly controlled. The differentiation degree can be observed under a microscope. You can differentiate for a few seconds first, rinse with water and observe under the microscope until the collagen fibers and muscle fibers are nearly colorless or light gray, and the myelin sheath shows clear blue.
2. If the differentiation process is difficult to control, the Page Differentiation Solution can be diluted 4-5 times with distilled water before differentiation.
3. The sections should not be too thick, and the thickness should be controlled within 5-6 μm; otherwise, section detachment or over-staining may occur.
4. New Page Fuchsine Staining Solution requires a relatively short staining time, while the staining ability of the stored solution for a long time will decrease, and it can be slightly warmed before staining for several minutes.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
6. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
砂罗铬花青髓鞘染色液
规格或纯度
BioReagent,用于显微镜,生物染色剂
英文名称
Modified Page Myelin Staining Solution
应用
细胞染色
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
各组分在相应的储存条件下保质期1年。
组分与储存
| I1508448 | Component | 4×50 mL | Storage |
| I1508448A | Page甲醛固定液 | 250 mL | RT |
| I1508448B | 改良Page染色液 | 50 mL | RT. Store in the dark. |
| I1508448C | Page分化液 | 50 mL | RT |
| I1508448D | Page桃红染色液 | 50 mL | RT. Store in the dark. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333022 |
分析证书 | I1508448 |
技术文档和文章
溶液计算器
常见问题
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
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