计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
苯酚品红染色液也叫石炭酸-品红染液,主要用于生物学上细胞分裂时染色体形态的染色剂,作为碱性染料,易溶于水、酒精、乙酸。
改良苯酚品红染色液,即卡宝品红(Carbol fuchsin)染色液,该染色液与醋酸洋红存在相似之处,醋酸洋红是一种较为常用的碱性染料,常用于细胞核染色、染色体的固定和染色。改良苯酚品红染色液对果蝇唾液腺染色体的染色效果与醋酸染洋红染色液的染色效果是相同的,用改良苯酚品红染色液还能提高工效,简易节约的优点;染色体染色时用醋酸洋红染色液染色,尽管染色效果较好,但却存在着染色需时较长的问题。
改良苯酚品红染色液常用于植物组织的压片法和涂片法,此液具有醋酸洋红染色方便的优点,还具有席夫试剂只对核和染色体染色的优点,且染色效果稳定可靠。此液适于动植物各种大小的染色体、体细胞染色体和减数分裂染色体,并具有相当牢固的染色性能,保存性好。该产品仅适用于科研实验,不可做他用。
自备仪器、耗材和试剂
操作步骤(仅供参考)
1、花粉类颗粒:取少量花粉物质置于载玻片,滴加适量改良苯酚品红染色液,充分混合,立即盖片染色5-15min后,吸去多余液体,显微镜下观察。
2、动植物切片:常规处理(如脱蜡至水),滴加改良苯酚品红染色液5-10min,蒸馏水冲洗,封片,显微镜下观察。
3、部分新鲜植物组织(如洋葱表皮),直接浸染于改良苯酚品红染色液5-10min, 蒸馏水冲洗2~3min,置于载玻片并盖上盖玻片,显微镜观察。
注意事项
1、染完色后,应立即显微镜下观察。
2、由于本试剂含有高浓度弱酸,有刺激性气味,请注意自我保护。
Phenol fuchsin staining solution, also known as carbolic acid-fuchsin stain, is primarily used in biology as a stain for observing chromosomal morphology during cell division. As a basic dye, it is readily soluble in water, alcohol, and acetic acid.
Modified Phenol Fuchsin Staining Solution, also referred to as Carbol fuchsin staining solution, shares similarities with acetocarmine stain. Acetocarmine is a commonly used basic dye often employed for nuclear staining, as well as for the fixation and staining of chromosomes. The staining effect of Modified Phenol Fuchsin Staining Solution on Drosophila salivary gland chromosomes is identical to that of acetocarmine. However, using Modified Phenol Fuchsin Staining Solution can improve efficiency and offers the advantages of simplicity and cost-effectiveness. While acetocarmine provides good staining results for chromosomes, it requires a longer staining time.
Modified Phenol Fuchsin Staining Solution is commonly used for squash and smear methods in plant tissue studies. This solution combines the convenience of acetocarmine staining with the specificity of Schiff's reagent, which stains only nuclei and chromosomes. It offers stable and reliable staining results, suitable for chromosomes of various sizes in both plants and animals, including somatic and meiotic chromosomes. The staining is durable and well-preserved. This product is intended for research and experimental use only and must not be used for other purposes.
Reagents, consumables and Equipments not provided
Glass slides and cover slips
Optical microscope
Procedure (For Reference Only)
Pollen or similar particles: Place a small amount of pollen material on a glass slide, add an appropriate amount of Modified Phenol Fuchsin Staining Solution, and mix thoroughly. Immediately cover with a cover slip and stain for 5–15 minutes. Absorb excess liquid and observe under a microscope.
Animal or plant tissue sections: After routine processing (e.g., deparaffinization to water), apply Modified Phenol Fuchsin Staining Solution for 5–10 minutes. Rinse with distilled water, mount the slide, and observe under a microscope.
Certain fresh plant tissues (e.g., onion epidermis): Directly immerse in Modified Phenol Fuchsin Staining Solution for 5–10 minutes. Rinse with distilled water for 2–3 minutes, place on a glass slide, cover with a cover slip, and observe under a microscope.
Precautions
After staining, observe immediately under a microscope.
As this reagent contains a high concentration of weak acid and has an irritating odor, please take necessary precautions for personal protection.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | I1508550 | |
| 分析证书 | I1508550 | |
| 分析证书 | I1508550 | |
| 分析证书 | I1508550 |
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