计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
异柠檬酸是柠檬酸的异构体,虽然量少,但广泛存在于生物界。在落地生根属等多汁植物的叶、或悬钩子类中特别多,也是三羧酸循环中的一个成分。 本试剂盒提供一种特异性酶法检测异柠檬酸含量,利用异柠檬酸脱氢酶使异柠檬酸和NADP⁺生成α-酮戊二酸、二氧化碳和NADPH,通过测定NADPH在340nm处吸光值的增加量,进而计算出样品中异柠檬酸含量。
Isocitrate is an isomer of citrate. Although present in small quantities, it is widely found in the biological world. It is particularly abundant in the leaves of succulent plants such as those from the genus Bryophyllum, or in species like Rubus, and it is also a component of the tricarboxylic acid cycle. This kit provides a specific enzymatic method for detecting isocitrate content. It utilizes isocitrate dehydrogenase to catalyze the conversion of isocitrate and NADP⁺ into α-ketoglutarate, carbon dioxide, and NADPH. By measuring the increase in absorbance of NADPH at 340 nm, the isocitrate content in the sample can be calculated.