计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
果胶是构成细胞初生壁和中胶层的主要成分,主要由原果胶、果胶酸甲酯和果胶酸组成。果胶中含有半乳糖醛酸、乳糖、阿拉伯糖、葡萄糖醛酸等,是许多高等植物细胞壁中含量最丰富的多糖成分,其独特的物理、化学性质影响着植物源食品的口感和品质。果胶间以Ca²⁺桥及其他离子键、氢键、糖苷键、酯键和苯环偶联的方式交联,通过不同的抽提方法可以提取各种形式的果胶,如水溶性果胶(WSP)、离子结合型果胶(ISP)和共价结合型果胶(CSP)。该产品提供了一种简单、方便、快速的 ISP 含量检测方法,适用于植物组织样本。其原理是利用带有螯合剂的酸溶液提取离子结合型果胶(ISP),采用咔唑比色法测定果胶含量。果胶水解成半乳糖醛酸,在硫酸溶液中与咔唑试剂进行缩合反应,生成物质在 530 nm 处有最大吸收峰。
Pectin is a major component of the primary cell wall and the middle lamella of plant cells, primarily composed of protopectin, pectin methylester, and pectic acid. Pectin contains galacturonic acid, galactose, arabinose, glucuronic acid, etc., and is the most abundant polysaccharide component in the cell walls of many higher plants. Its unique physical and chemical properties influence the taste and quality of plant-derived foods. Pectin molecules are cross-linked through Ca²⁺ bridges, as well as other ionic bonds, hydrogen bonds, glycosidic bonds, ester bonds, and benzene ring coupling. Various forms of pectin can be extracted using different extraction methods, such as water-soluble pectin (WSP), ion-bound pectin (ISP), and covalently-bound pectin (CSP). This product provides a simple, convenient, and rapid method for determining ISP content, suitable for plant tissue samples. Its principle involves extracting ion-bound pectin (ISP) using an acidic solution containing a chelating agent, followed by the carbazole colorimetric method for pectin quantification. Pectin is hydrolyzed to galacturonic acid, which undergoes a condensation reaction with carbazole reagent in a sulfuric acid solution. The resulting compound has a maximum absorption peak at 530 nm.