计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
植物体内生长素的种类很多,其中吲哚乙酸(IAA)是植物体内普遍存在的一种生长素,植物体内IAA的含量,对于植物的生长、发育、衰老、脱落等均有重要意义。植物体内存在吲哚乙酸氧化酶(Indoleacetic acid oxida-se),该酶是植物体内一种氧化分解吲哚乙酸的酶,吲哚乙酸氧化酶能够氧化IAA使其失去活性,从而调节体内IAA的水平,影响植物的生长。 该试剂盒检测原理是吲哚乙酸在吲哚乙酸氧化酶作用下形成吲哚醛,使体系中吲哚乙酸含量减少,剩余的吲哚乙酸在无机酸存在下与FeCl₃作用生成红色螯合物,吲哚乙酸氧化酶活性的大小可以用其破坏吲哚乙酸的速度表示,通过测定530nm处吸光度,根据对照与待测样品中吲哚乙酸含量的差值,计算出吲哚乙酸氧化酶活性水平,主要用于检测植物样本、血清等中吲哚乙酸氧化酶活性,尤其适用于定量测定植物样本吲哚乙酸氧化酶活性。本产品仅用于科研领域,不适用于临床诊断或其他用途。
There are many types of auxins in plants, among which indole-3-acetic acid (IAA) is a commonly occurring auxin. The content of IAA in plants is significant for processes such as growth, development, aging, and abscission. Plants contain indoleacetic acid oxidase, an enzyme that oxidatively degrades IAA. This enzyme can oxidize IAA, rendering it inactive, thereby regulating the endogenous IAA level and influencing plant growth. The detection principle of this kit is as follows: Indole-3-acetic acid is converted into indolealdehyde by the action of indoleacetic acid oxidase, reducing the IAA content in the system. The remaining IAA reacts with FeCl₃ in the presence of a mineral acid to form a red chelate. The activity of indoleacetic acid oxidase can be determined by the rate at which it degrades IAA. By measuring the absorbance at 530 nm and comparing the difference in IAA content between the control and the test sample, the activity level of indoleacetic acid oxidase can be calculated. This kit is primarily used for detecting indoleacetic acid oxidase activity in plant samples, serum, etc., and is particularly suitable for the quantitative determination of indoleacetic acid oxidase activity in plant samples. This product is intended for research use only and is not suitable for clinical diagnostic or other purposes.