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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
ICL(EC4.1.3.1)主要存在于植物和微生物中,油料作物种子在萌发过程中,通过乙醛酸循环及其他过程将脂肪转变成碳水化合物。ICL是乙醛酸循环的关键酶之一。 测定原理:ICL催化异柠檬酸降解为乙醛酸和琥珀酸,乙醛酸和NADH在LDH的作用下生成乙醇和NAD,NADH在340nm下有特征吸收峰,监测340nm吸光度的减小速率可间接反应ICL活性。
ICL (EC 4.1.3.1) is mainly found in plants and microorganisms. During the germination of oilseed crops, fats are converted into carbohydrates through the glyoxylate cycle and other processes. ICL is one of the key enzymes in the glyoxylate cycle. Determination Principle: ICL catalyzes the degradation of isocitrate to glyoxylate and succinate. Glyoxylate and NADH are then converted to ethanol and NAD⁺ in the presence of LDH. NADH exhibits a characteristic absorption peak at 340 nm, and the ICL activity can be indirectly determined by monitoring the rate of decrease in absorbance at 340 nm.