IMDM（Iscove’s Modified Dulbecco Medium）即Iscove改良的DMEM培养基，是Guilber 和Iscove在1976年设计的，用以培养红细胞前体细胞和巨噬细胞。IMDM培养基在DMEM培养基的基础上添加了硒、HEPES、丙酮酸钠以及额外的氨基酸和维生素，并用硝酸钾代替了硝酸铁，营养非常丰富，非常适合于快速增殖，高密度细胞培养。IMDM培养基不仅可以培养有特殊营养要求的细胞（如小鼠B淋巴细胞，LPS刺激的B细胞，骨髓造血细胞，T淋巴细胞以及各种杂交瘤细胞），还可以作为一些独特的无血清培养基的基础液。青霉素-链霉素混合液通常也被称为“双抗”，是体外培养中为预防微生物污染最常用的抗生素，其中，青霉素能够干扰细菌细胞壁的合成，对革兰阳性菌特别有效；而链霉素能够与细菌核糖体30S亚单位结合，抑制细菌蛋白质的合成，对革兰氏阴性菌和革兰氏阳性菌均有效，但对革兰氏阴性菌特别有效。青霉素和链霉素联合使用可以预防绝大部分的细菌污染。本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分；但不含蛋白质、脂类或任何生长因子；故此产品需搭配血清或无血清添加物使用。注意事项 1、本产品经过滤除菌，使用时应注意无菌操作，避免污染； 2、为保持本产品的使用效果，请勿进行冻融处理； 3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。

IMDM (Iscove’s Modified Dulbecco Medium) was developed by Guilber and Iscove in 1976 for the culture of erythroid precursor cells and macrophages. Based on DMEM, IMDM is supplemented with selenium, HEPES, sodium pyruvate, and additional amino acids and vitamins, and replaces ferric nitrate with potassium nitrate. It is nutritionally rich and highly suitable for rapidly proliferating, high‑density cell cultures. IMDM can be used not only for cells with special nutritional requirements (such as mouse B lymphocytes, LPS‑stimulated B cells, bone marrow hematopoietic cells, T lymphocytes, and various hybridoma cells), but also serves as a basal medium for some unique serum‑free formulations. Penicillin‑streptomycin mixture, commonly referred to as “dual antibiotics” (or “pen‑strep”), is the most frequently used antibiotic combination for preventing microbial contamination in in vitro culture. Penicillin interferes with bacterial cell wall synthesis and is particularly effective against Gram‑positive bacteria, while streptomycin binds to the 30S ribosomal subunit, inhibiting bacterial protein synthesis, and is effective against both Gram‑negative and Gram‑positive bacteria, with particular potency against Gram‑negative organisms. The combined use of penicillin and streptomycin can prevent the majority of bacterial contamination. This product contains multiple components required for cell culture, including amino acids, vitamins, inorganic salts, etc.; however, it contains no proteins, lipids, or any growth factors. Therefore, this product must be used with serum or serum‑free supplements. Precautions 1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. 2. To maintain optimal performance, avoid freeze‑thaw cycles. 3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.