IMDM（Iscove’s Modified Dulbecco Medium）即Iscove改良的DMEM培养基，是Guilber 和Iscove在1976年设计的，用以培养红细胞前体细胞和巨噬细胞。IMDM培养基在DMEM培养基的基础上添加了硒、HEPES、丙酮酸钠以及额外的氨基酸和维生素，并用硝酸钾代替了硝酸铁，营养非常丰富，非常适合于快速增殖，高密度细胞培养。IMDM培养基不仅可以培养有特殊营养要求的细胞（如小鼠B淋巴细胞，LPS刺激的B细胞，骨髓造血细胞，T淋巴细胞以及各种杂交瘤细胞），还可以作为一些独特的无血清培养基的基础液。
酚红在培养基中被用来作为PH值的指示剂，用以持续监测培养液的酸碱度，在低PH值时酚红使培养液呈黄色，而较高的PH值时使培养液呈紫色，PH值7.2～7.4时为红色，最适合细胞培养。但酚红也有一些缺点，研究表明酚红可以模拟固醇类激素（特别是雌激素）的作用，因此在用到雌激素敏感的细胞时（如乳腺组织），最好使用不含酚红的培养基。酚红会干扰流式细胞分析时候的检测。此外，一些无血清培养基的配方中若存在酚红会干扰钠-钾平衡。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分；但不含蛋白质、脂类或任何生长因子；故此产品需搭配血清或无血清添加物使用。
注意事项
1、本产品经过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
IMDM (Iscove’s Modified Dulbecco Medium) was developed by Guilber and Iscove in 1976 for the culture of erythroid precursor cells and macrophages. Based on DMEM, IMDM is supplemented with selenium, HEPES, sodium pyruvate, and additional amino acids and vitamins, and replaces ferric nitrate with potassium nitrate. It is nutritionally rich and highly suitable for rapidly proliferating, high‑density cell cultures. IMDM can be used not only for cells with special nutritional requirements (such as mouse B lymphocytes, LPS‑stimulated B cells, bone marrow hematopoietic cells, T lymphocytes, and various hybridoma cells), but also serves as a basal medium for some unique serum‑free formulations.
Phenol red is used in the medium as a pH indicator to continuously monitor the acidity/alkalinity of the culture fluid. At low pH, phenol red turns the medium yellow; at high pH, it turns purple; at pH 7.2–7.4, it appears red, which is optimal for cell culture. However, phenol red has some drawbacks. Studies have shown that phenol red can mimic the action of steroid hormones, particularly estrogens. Therefore, when using estrogen‑sensitive cells (e.g., breast tissue), it is advisable to use phenol‑red‑free medium. Phenol red can also interfere with flow cytometry analysis. In addition, the presence of phenol red in some serum‑free formulations may disturb the sodium‑potassium balance.
This product contains multiple components required for cell culture, including amino acids, vitamins, inorganic salts, etc.; however, it contains no proteins, lipids, or any growth factors. Therefore, this product must be used with serum or serum‑free supplements.
Precautions
1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination.
2. To maintain optimal performance, avoid freeze‑thaw cycles.
3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.
危险品化学品经营许可证（带存储）