BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α-酮戊二酸（α-ketoglutarate，α-KG）是三羧酸循环中的重要中间代谢物，位于异柠檬酸之后、琥珀酰辅酶A之前，连接碳代谢与氮代谢，不仅直接参与机体氧化供能，还参与多种物质的生物合成，对维持机体正常生理功能具有重要作用。本试剂盒提供一种快速、灵敏的α-酮戊二酸检测方法：在谷丙转氨酶催化下，α-KG转化为谷氨酸；谷氨酸在谷氨酸脱氢酶作用下进一步反应生成NADH；生成的NADH与显色剂反应，产生在450 nm处具有特征吸收峰的黄色产物。通过测定该波长下吸光值的生成量，即可精准计算样品中α-KG的含量。
α-Ketoglutarate (α-KG) is an important intermediate metabolite in the tricarboxylic acid (TCA) cycle, positioned after isocitrate and before succinyl-CoA. It links carbon and nitrogen metabolism, directly participating in oxidative energy production and the biosynthesis of various substances, thus playing a vital role in maintaining normal physiological functions. This kit provides a rapid and sensitive method for the determination of α-KG: Under the catalysis of alanine aminotransferase (ALT), α-KG is converted to glutamate. The glutamate is then further reacted by glutamate dehydrogenase (GDH) to generate NADH. The generated NADH reacts with a chromogenic reagent to produce a yellow product with a characteristic absorbance peak at 450 nm. By measuring the absorbance at this wavelength, the α-KG content in the sample can be precisely calculated.
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