计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
K6PC-5, a ceramide derivative, is a sphingosine kinase 1(SPHK1) activator and elicites a rapid transient increase in intracellular calcium levels. K6PC-5 has the potential for skin diseases involving abnormal keratinocyte, and neurodegeneration and virus infection research
In Vitro
K6PC-5 (1-10 μM; 24 h) increases the involucrin and loricrin levels in a dose-dependent manner in normal human epidermal keratinocytes (NHEKs). K6PC-5 promotes differentiation and proliferation of keratinocytes via intracellular Ca 2+ signaling. In addition, K6PC-5 stimulates the phosphorylation of p42/44 extracellular signal-regulated kinase and c-Jun N-terminal kinase. K6PC-5 (1-10 μM; 24 h) promotes fibroblasts proliferation and collagen synthesis in human fibroblasts. K6PC-5 induces intracellular Ca 2+ concentration ([Ca 2+ ] i ) oscillations in human fibroblasts. K6PC-5 (10, 25, and 50 μM; 48 h) significantly attenuates EBOV-induced infection in EBOV-infected EA.hy926 cells. K6PC-5 significantly reduces the virus titers in supernatants of infected cells and strikingly decreased the amount of VP40 in a concentration-dependent manner. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: Normal human epidermal keratinocytes (NHEKs) Concentration: 1 μM, 5 μM, 10 μM Incubation Time: 24 h Result: Increased the involucrin and loricrin levels in a dose-dependent manner. Cell Proliferation AssayCell Line: Human fibroblasts Concentration: 1 μM, 5 μM, 10 μM Incubation Time: 24 h Result: Promoted fibroblast proliferation and procollagen production in human fibroblasts significantly.
In Vivo
In intrinsically aged hairless mice (56 weeks old), 1% K6PC-5 is applied topically for 2 weeks. This K6PC-5 treatment significantly increases both the number of dermal fibroblasts and collagen production. As a consequence, dermal thickness also increased significantly. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Intrinsically aged hairless mice (56 weeks old)Dosage: 1% (vehicle (PEG:EtOH = 7:3)) Administration: Topical application; twice daily for 2 weeks Result: Enhanced fibroblast proliferation, collagen production, and eventually increased dermal thickness.
IC50& Target:SphK1
规格
规格或纯度
10mM in DMSO
英文名称
K6PC-5
生化机理
K6PC-5 是一种神经酰胺衍生物，是一种鞘氨醇激酶 1（SPHK1）激活剂，可引起细胞内钙水平的快速瞬时升高。K6PC-5 具有治疗涉及异常角质细胞的皮肤病、神经变性和病毒的潜力。
储存条件
-80℃储存
运输条件
特低温运输
作用类型
抑制剂
名称和识别符
技术文档
高级数据
安全和危险性（GHS）
RIDADR
NONHforallmodesoftransport
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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溶液计算器
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