What Is C2orf78? Gene and Protein Background

C2orf78, or Chromosome 2 Open Reading Frame 78, is a human gene located on chromosome 2 that encodes a protein whose precise biological function remains under active investigation. Proteins designated as open reading frames (ORFs) represent an important frontier in proteomics: they are confirmed to be expressed at the transcript and/or protein level, yet their molecular roles, interaction partners, and cellular localizations have not been fully characterized in the literature.

Recombinant expression of such proteins, as offered by Aladdin Scientific (SKU: rp376447), enables researchers to study their biochemical properties directly, screen for interacting partners, generate antibodies, and begin the process of functional annotation. Understanding uncharacterized ORF proteins is critical for building a complete picture of the human proteome and may have downstream relevance to disease biology as functional studies progress.

Production and Biochemical Properties

Recombinant Human C2orf78 Protein (rp376447) is produced using recombinant protein expression technology. Recombinant proteins of this class are typically expressed in a suitable host system — commonly Escherichia coli, insect cells (baculovirus), or mammalian cell lines — followed by affinity chromatography purification to achieve research-grade purity. The choice of expression system can influence post-translational modifications, folding, and solubility of the final protein product.

Researchers should consult the official product datasheet for rp376447 (referenced as absea#PP-10008 in supplier documentation) for confirmed specifications including molecular weight, purity grade, formulation buffer, storage conditions, and recommended handling instructions. Key quality attributes to verify include: SDS-PAGE purity confirmation, endotoxin level, and protein concentration upon reconstitution.

Proper storage — typically at −20°C or −80°C with avoidance of repeated freeze-thaw cycles — is essential to maintain protein activity and integrity over time.

Research Applications

Recombinant Human C2orf78 Protein is suitable for a range of in vitro research applications commonly used to characterize novel or poorly annotated proteins:

• Protein–protein interaction studies: Co-immunoprecipitation, pull-down assays, and yeast two-hybrid screens can be used to identify binding partners of C2orf78, providing clues to its cellular function.
• Antibody development and validation: Recombinant protein serves as an antigen for immunizing animals to generate polyclonal or monoclonal antibodies, or as a positive control in Western blotting and ELISA assays.
• Structural studies: Purified recombinant C2orf78 can be used in circular dichroism (CD) spectroscopy or X-ray crystallography attempts to determine secondary and tertiary structure.
• Functional assays: Enzyme activity assays or cell-based assays may be employed once putative functions are hypothesized through bioinformatics analysis of sequence homology.
• Proteomics workflows: The recombinant protein can serve as a reference standard in mass spectrometry-based proteomics experiments.

Because C2orf78 remains incompletely characterized, researchers working with this protein are encouraged to cross-reference results with transcriptomic databases such as GTEx and protein databases such as UniProt to contextualize experimental findings.

Handling, Storage, and Quality Considerations

To ensure optimal performance of Recombinant Human C2orf78 Protein (rp376447), researchers should adhere to the following general best practices for recombinant proteins:

• Storage temperature: Store at the temperature specified on the product datasheet, typically −20°C for short-term or −80°C for long-term storage.
• Freeze-thaw cycles: Minimize the number of freeze-thaw cycles by preparing single-use aliquots upon first thaw.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified in the product datasheet. Allow the vial to equilibrate to room temperature before opening.
• Protein stability: Addition of a carrier protein (e.g., 0.1% BSA) may be recommended for highly dilute working solutions to prevent adsorption to tube surfaces.
• Endotoxin levels: For cell-based assays, confirm that endotoxin levels are within acceptable limits as stated on the Certificate of Analysis (CoA).

Always consult the Certificate of Analysis supplied with each lot of rp376447 for lot-specific data on purity, concentration, and any additional quality control tests performed.

Ordering and Support

Recombinant Human C2orf78 Protein is available from Aladdin Scientific under SKU rp376447. Researchers can place orders directly through the Aladdin Scientific website or by contacting the sales team. For bulk quantities, custom formulations, or specific quality requirements, please reach out to Aladdin Scientific customer support.

Technical support is available for questions regarding reconstitution protocols, recommended assay conditions, and compatibility of the protein with specific experimental workflows. When contacting support, please have your lot number and Certificate of Analysis on hand to facilitate efficient troubleshooting.

For the most current pricing, availability, and detailed product specifications, visit the product page for rp376447 on the Aladdin Scientific website.