What Is FRMD4B? Gene and Protein Background

FRMD4B (FERM Domain-Containing Protein 4B) is a member of the Band 4.1 superfamily, a large group of proteins characterized by the presence of a FERM (4.1, Ezrin, Radixin, Moesin) domain. The FERM domain is a conserved structural module that typically mediates interactions between cytoskeletal components and the plasma membrane, playing critical roles in organizing membrane-associated protein complexes.

The human FRMD4B gene encodes a protein implicated in the regulation of epithelial cell polarity, vesicular trafficking, and cortical cytoskeleton organization. FRMD4B has been identified as a binding partner of cytohesin ARF-GEFs (guanine nucleotide exchange factors), linking it to ARF GTPase signaling pathways that control membrane dynamics and cell adhesion. Research has also connected FRMD4B to neurological contexts, including potential roles in Alzheimer's disease susceptibility based on genome-wide association studies.

As a recombinant human protein, rp365461 from Aladdin Scientific provides researchers with a well-defined tool to investigate FRMD4B's molecular functions, binding partners, and regulatory roles in vitro and in cell-based assay systems.

Biochemical Properties and Formulation

Recombinant Human FRMD4B Protein (rp365461) is produced using recombinant DNA technology to ensure high consistency and reproducibility across experiments. As with all recombinant proteins in Aladdin Scientific's catalog, this product is manufactured and quality-controlled to meet the demands of rigorous biochemical and cell-biological research.

Key biochemical considerations for working with FRMD4B protein include:

• Domain architecture: FRMD4B contains an N-terminal FERM domain responsible for membrane and cytoskeletal interactions, along with additional C-terminal regulatory regions.
• Solubility: FERM domain proteins may require optimized buffer conditions for maximal solubility; researchers should refer to the product datasheet for recommended reconstitution and storage buffers.
• Storage: Recombinant proteins should generally be stored at -80 °C upon receipt and avoid repeated freeze-thaw cycles to maintain activity and integrity.
• Purity: Research-grade recombinant proteins are typically validated by SDS-PAGE and, where applicable, functional assays.

Note: Specific molecular weight, purity percentage, endotoxin levels, and formulation buffer details should be confirmed on the official product datasheet (absea#PP-1031) or by contacting Aladdin Scientific technical support.

Research Applications of Recombinant Human FRMD4B Protein

Recombinant Human FRMD4B Protein is a versatile reagent suitable for multiple experimental workflows:

• Protein-protein interaction studies: FRMD4B's FERM domain facilitates binding to cytoskeletal and membrane proteins. Recombinant FRMD4B can be used in co-immunoprecipitation, pull-down assays, or surface plasmon resonance (SPR) experiments to map interaction networks.
• Biochemical activity assays: Researchers investigating ARF-GEF regulation and membrane dynamics can use recombinant FRMD4B to study how it modulates cytohesin family proteins in cell-free systems.
• Antibody development and validation: Recombinant proteins serve as reliable antigens for antibody production and as positive controls in Western blot, ELISA, and immunofluorescence experiments.
• Structural biology: Purified recombinant FRMD4B can be used in structural studies such as X-ray crystallography or cryo-electron microscopy to elucidate domain architecture and conformational dynamics.
• Drug discovery screening: FRMD4B is a potential target in disease contexts; recombinant protein enables development of binding assays for small-molecule or peptide inhibitor screening.
• Cell polarity research: Given FRMD4B's role in epithelial polarity, recombinant protein supports mechanistic dissection of polarity complex assembly and regulation.

Ordering, Storage, and Technical Support

Recombinant Human FRMD4B Protein is available from Aladdin Scientific under SKU rp365461 (supplier reference: absea#PP-1031). To order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

Storage recommendations (general guidelines for recombinant proteins):

• Upon receipt, briefly centrifuge the vial before opening.
• Aliquot into single-use volumes to minimize freeze-thaw cycles.
• Store at -80 °C for long-term storage; -20 °C for short-term use (up to 1 month).
• Avoid repeated freeze-thaw cycles, which can compromise protein integrity and activity.
• Reconstitute lyophilized protein according to the datasheet instructions using the recommended buffer.

Technical support: For specific questions regarding protein activity, reconstitution protocols, or experimental design using rp365461, contact the Aladdin Scientific technical support team. Product-specific certificates of analysis (CoA) and safety data sheets (SDS) are available upon request.