Recombinant Human HSP90AA2P Protein: Research Guide
Recombinant Human HSP90AA2P Protein: Research Guide
Recombinant Human HSP90AA2P Protein is a recombinant form of the human HSP90AA2P gene product, offered by Aladdin Scientific (SKU: rp370177) to support research into heat shock protein biology, molecular chaperone pathways, and cellular stress response mechanisms.
- Product Name
- Recombinant Human HSP90AA2P Protein
- SKU
- rp370177
- Protein Family
- HSP90 heat shock protein family
- Species Origin
- Human (Homo sapiens)
- Application
- Research use only; molecular chaperone and stress-response studies
- Storage Recommendation
- Store at -80 °C; avoid repeated freeze-thaw cycles (refer to CoA for confirmed conditions)
Biological Background of HSP90AA2P
HSP90AA2P is a gene locus in the human genome that belongs to the HSP90 (Heat Shock Protein 90) superfamily. Heat shock proteins are a conserved family of molecular chaperones that play essential roles in protein folding, assembly, stabilization, and degradation under both normal physiological conditions and cellular stress. The HSP90 family, in particular, is critical in maintaining proteostasis and supporting the maturation of numerous client proteins involved in signal transduction, cell cycle regulation, and apoptosis.
HSP90AA2P has been annotated as a pseudogene-related locus; however, recombinant proteins derived from such loci are valuable tools for investigating gene expression nuances, potential protein products, and their interactions within the broader HSP90 network. Understanding HSP90AA2P contributes to a more complete picture of heat shock protein diversity in human cells.
Product Specifications and Quality
Aladdin Scientific's Recombinant Human HSP90AA2P Protein (SKU: rp370177) is produced using recombinant expression systems to ensure batch-to-batch consistency suitable for demanding research applications. Key quality attributes to confirm on the Certificate of Analysis (CoA) include:
- Purity: Assessed by SDS-PAGE and/or HPLC; typical target purity for recombinant proteins of this class is ≥85% (verify exact specification on CoA).
- Formulation: Typically supplied in a buffered solution optimized for protein stability; exact buffer composition and concentration are provided on the product datasheet.
- Endotoxin Level: Tested by LAL assay; low endotoxin is critical for cell-based assays (confirm limits on CoA).
- Biological Activity: Confirm functional validation method on the product datasheet.
All specifications should be verified against the current Certificate of Analysis supplied with each lot.
Handling, Reconstitution, and Storage Protocol
Proper handling of Recombinant Human HSP90AA2P Protein is essential to preserve its integrity and activity throughout your experiments. Follow these guidelines:
- Receipt: Upon receipt, immediately inspect packaging integrity and transfer the protein to the recommended storage temperature. Do not leave at room temperature for extended periods.
- Reconstitution: If supplied as a lyophilized powder, gently centrifuge the vial before opening. Reconstitute with the volume of sterile ultrapure water or recommended buffer as specified on the datasheet. Allow the solution to stand for a few minutes and mix gently by pipetting; do not vortex.
- Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize freeze-thaw cycles, which can degrade protein activity.
- Storage: Store aliquots at -80 °C for long-term preservation. For short-term use (up to 1 week), -20 °C may be appropriate depending on formulation (confirm on datasheet).
- Freeze-Thaw: Limit freeze-thaw cycles to no more than 2–3. Each additional cycle may result in protein aggregation and loss of activity.
Always refer to the product-specific datasheet and CoA for definitive handling instructions.
Research Applications
Recombinant Human HSP90AA2P Protein is intended for research use only and may be employed in a variety of experimental contexts related to heat shock protein biology:
- Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays or co-immunoprecipitation experiments to identify binding partners within the HSP90 interactome.
- Antibody Development and Validation: Serve as a positive control antigen for validating anti-HSP90AA2P antibodies in Western blot, ELISA, and immunofluorescence.
- Structural and Biochemical Characterization: Supports biophysical studies such as circular dichroism (CD) spectroscopy or thermal shift assays to assess protein folding and stability.
- Functional Assays: Investigate potential chaperone activity or client protein interactions in in vitro reconstitution systems.
- Drug Discovery: Use as a target protein in small-molecule binding assays relevant to HSP90 inhibitor research.
This product is not intended for diagnostic, therapeutic, or in vivo use.
Troubleshooting Common Issues
Researchers may encounter the following issues when working with Recombinant Human HSP90AA2P Protein. The guidance below provides general best-practice solutions:
| Issue | Possible Cause | Recommended Action |
|---|---|---|
| Protein precipitation or aggregation | Improper reconstitution, excess freeze-thaw cycles, or incompatible buffer | Reconstitute as directed; minimize freeze-thaw; centrifuge at 10,000×g for 5 min before use to remove aggregates |
| Low or undetectable activity | Protein degradation due to improper storage or expired lot | Verify storage conditions and lot expiry; use freshly thawed aliquots; include positive controls |
| High background in ELISA or Western blot | Non-specific antibody binding or excess protein loading | Optimize antibody dilutions and blocking conditions; titrate protein concentration |
| Unexpected band size on SDS-PAGE | Post-translational modifications, tags, or degradation fragments | Consult CoA for expected molecular weight; verify reducing vs. non-reducing conditions |
Frequently asked questions
What is HSP90AA2P and why is it relevant for research?
HSP90AA2P is a locus in the human genome belonging to the HSP90 heat shock protein superfamily. Recombinant proteins derived from this locus are used to study molecular chaperone networks, protein–protein interactions within the HSP90 interactome, and stress-response pathways. It may also serve as an antigen for antibody validation.
How should Recombinant Human HSP90AA2P Protein be stored to maintain stability?
For long-term storage, keep the protein at -80 °C in single-use aliquots to avoid repeated freeze-thaw cycles. Short-term storage at -20 °C may be acceptable depending on the formulation. Always consult the product-specific datasheet and CoA for confirmed storage conditions and shelf life.
What are the recommended applications for this recombinant protein?
Recombinant Human HSP90AA2P Protein (rp370177) is suitable for protein–protein interaction studies, antibody development and validation (Western blot, ELISA, immunofluorescence), structural characterization, in vitro functional assays, and small-molecule binding studies in drug discovery contexts. It is for research use only.
How do I minimize protein aggregation during reconstitution?
Gently centrifuge the vial before opening, then reconstitute with the recommended buffer as specified on the datasheet. Mix by gentle pipetting rather than vortexing. Prepare single-use aliquots immediately and store at -80 °C. Prior to use, centrifuge the thawed protein at 10,000×g for 5 minutes to remove any aggregates.
Is Recombinant Human HSP90AA2P Protein suitable for in vivo or clinical use?
No. This product is strictly for research use only and is not approved or intended for diagnostic, therapeutic, or any in vivo application in humans or animals. Researchers must comply with all applicable institutional and regulatory requirements when handling recombinant proteins.
References
- Taipale M, Jarosz DF, Lindquist S. HSP90 at the hub of protein homeostasis: emerging mechanistic insights. Nature Reviews Molecular Cell Biology. 2010;11(7):515–528. doi:10.1038/nrm2918
- Röhl A, Rohrberg J, Buchner J. The chaperone Hsp90: changing partners for demanding clients. Trends in Biochemical Sciences. 2013;38(5):253–262. doi:10.1016/j.tibs.2013.02.003
- Aladdin Scientific. Recombinant Human HSP90AA2P Protein, SKU rp370177. Product Datasheet. Aladdin Scientific.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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