实验方案（Protocols）
Experiments for the determination of luciferase
Experiments for the determination of luciferase
Detection of Firefly Luciferase Activity by Using Luciferin as a Substrate
Principle
Fluorescein + ATP + O2 → oxygen and worm fluorescein + PPi + H2O + light The optical density I is related to the Michaelis-Menten equation where H is the Planck constant and v is the frequency of emitted light. When the ATP concentration is very low ([ATP] < < Km), the Michaelis-Menten equation simplifies to: I = Constant × [ATP] Constant = V/Km. optical density is proportional to [ATP]. Appliance cell-based assay For more product details, please visit Aladdin Scientific website.Luciferin 4-monooxygenase (ATP-hydrolyzed) extracted from firefly, Photinus Pyralis.
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