青霉素酶液体

Name: 青霉素酶液体
SKU: L140135
Availability: InStock

CAS: 9001-74-5 货号: L140135 EC号: 232-628-8

6篇引用文献 COA

有货

级别和纯度: 生物活性 ? 重组 ? ActiBioPure™ ? 高性能 ? EnzymoPure™ ? 无菌 ? expressed in E.coli,＞300万单位/1ml

Accession #

P00809

表达系统

E. coli

生物活性

≥3000000 U/mL

规格

库存

价格

数量

5ml

L140135-5ml

现货

¥92.90

8×5ml

L140135-8×5ml

现货

¥391.90

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概述规格储存与运输技术文档高级数据关联靶点（其它种属）作用机制质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,无菌,生物活性,重组,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存,充氩。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 6 篇同行评审文献引用。

概述

青霉素酶（β-内酰胺酶Ⅰ）（penicillinase）属β-内酰胺酶的一种。本公司采用生物技术，通过色谱层析技术分离纯化，获得了高效的青霉素酶。其具有纯度高、活性高、专一性强等特点。我们采用反相高效液相色谱法（RP-HPLC）、分子筛高效液相色谱法（SEC-HPLC）及还原和非还原电泳法（reducing and non-reducing SDS-PAGE）等三种方法对其纯度进行分析，其结果均达到 90%以上。

应用

1、可以有效水解的青霉素类抗生素包括：青霉素钠、氨苄西林、阿莫西林、匹氨西林、羧基青霉素类，如羧苄西林、替卡西林等；脲基青霉素类，如呋苄西林、美洛西林、头孢一代。

2 、注射用青霉素类抗生素药品无菌检测。

3 、口服青霉素类抗生素药品检验中的微生物限度检测、控制菌检查。

4 、本品对含酶抑制剂（克拉维酸钾、舒巴坦、他唑巴坦）的复方制剂（如阿莫西林克拉维酸钾）无效。

5、青霉素类制剂线模拟灌装消除管道中抗生素的残留亦可以使用（仅限于培养基灌装后培养前加入）。

6、环境监控培养基平皿中加入青霉素酶可降低假阴性风险，具体每个平皿的加酶量以验证为准。

使用方法

1、本品在进入无菌室前，请用消毒剂擦拭西林瓶的外壁，隔离器内表面灭菌适用。

2、可培养基、冲洗液、生理盐水复溶（液体无需复溶）。

3、冲洗液中加酶：使用无菌针筒或其他方法抽取酶液适量，加入灭菌的冲洗液中。

4、培养基中加酶：利用全封闭集菌培养器上自带的针头，抽取酶液适量，分别加入集菌器的各管中，酶可在导入培养基前加入，也可在导入培养基后加入。

建议用量

每批样品使用青霉素酶≥1500万单位，根据检验产品的抑菌性强弱、冲洗量适当调整加酶量，具体以方法学验证为准。

Penicillinase (β-lactamase Type I) is a member of the β-lactamase family. Our company has obtained high-efficiency penicillinase through biotechnological fermentation followed by chromatographic separation and purification. This product is characterized by high purity, high activity and strong specificity. Its purity was analyzed by three methods, namely reverse-phase high-performance liquid chromatography (RP-HPLC), size-exclusion high-performance liquid chromatography (SEC-HPLC) and reducing and non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purity determined by all the above methods is over 90%.

Applications

1. It can effectively hydrolyze the following penicillins: sodium penicillin, ampicillin, amoxicillin, pivampicillin, carboxypenicillins (e.g., carbenicillin, ticarcillin) and ureidopenicillins (e.g., furbenicillin, mezlocillin). Note: First-generation cephalosporins are not penicillins and are not hydrolyzed by this enzyme.

2. Sterility testing of injectable penicillin antibiotics.

3. Microbial limit testing and control bacteria examination in the quality inspection of oral penicillin antibiotics.

4. This product is ineffective against compound preparations containing β-lactamase inhibitors (e.g., clavulanate potassium, sulbactam, tazobactam), such as amoxicillin-clavulanate potassium.

5. It can also be used to eliminate antibiotic residues in pipelines during the simulated filling process of penicillin formulation production lines (only to be added after medium filling and before incubation).

6. Adding penicillinase to petri dishes containing environmental monitoring medium can reduce the risk of false negatives. The specific enzyme dosage per petri dish shall be subject to validation results.

Usage Instructions

1. Before entering the sterile room, wipe the outer wall of the vial with a disinfectant. This operation is also applicable to the surface sterilization of isolators.

2. The lyophilized powder can be reconstituted with culture medium, rinsing solution or normal saline (liquid formulations do not require reconstitution).

3. Adding enzyme to rinsing solution: Use a sterile syringe or other sterile devices to draw an appropriate amount of enzyme solution and add it to the sterilized rinsing solution.

4. Adding enzyme to culture medium: Use the built-in needle of the fully enclosed bacteria collection incubator to draw an appropriate amount of enzyme solution, and add it separately to each tube of the incubator. The enzyme can be added either before or after introducing the culture medium.

Recommended Dosage

The dosage of penicillinase for each batch of samples shall be not less than 15 million units. The dosage can be appropriately adjusted according to the antibacterial activity of the tested product and the volume of rinsing solution. The specific dosage shall be subject to methodological validation.

规格

产品名称

青霉素酶液体

别名

青霉素酶 | I型β-内酰胺酶 | β-内酰胺酶 I

英文别名

(6R,7R)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | CENTA | CENTA(TM) BETA-LACTAMASE SUBSTRATE | (6r,7r)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2

规格或纯度

生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,无菌,expressed in E.coli,＞300万单位/1ml

生化机理

This protein is a beta-lactamase with a substrate specificity for penicillins. The class A beta-lactamase family has a specific amino-acid numbering system, sometimes called Ambler or ABL numbering and often misspelt as Amber. A multiple sequence alignment was used to derive a consensus sequence and then the consensus was numbered taking into account insertions and deletions. This allows use of identical numbers, e.g. for active site residues, despite differences in protein length. UniProt always uses natural numbering of residues, hence there appear to be differences in numbering between this entry and some papers.

生物活性

≥3000000 U/mL

表达系统

E. coli

来源

重组表达

CAS编号和信息

9001-74-5

酶学委员会编号

3.5.2.6

分子类型

酶

储存与运输

物理形态

液体

浓度

expressed in E.coli,＞300万单位/1ml

储存条件

2-8°C储存,充氩

运输条件

低温运输

单位定义

PH7.0，37℃条件下，每小时分解青霉素钠活性单位的量。本品的活性测定参考《中国药典》收载的“青霉素酶活力测定法”。

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点（其它种属）

blaZ Beta-lactamase (285 活性数据)

点击查看靶蛋白信息： blaZ

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

bla OXA-48 (65 活性数据)

点击查看靶蛋白信息： bla

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到9个结果

批号(Lot Number)	证书类型	货号
G2523422	分析证书	L140135
H2308907	分析证书	L140135
B2602319	分析证书	L140135
E2022098	分析证书	L140135
B2320323	分析证书	L140135
B2320333	分析证书	L140135
L22071131	分析证书	L140135
I2208973	分析证书	L140135
C2226298	分析证书	L140135