计算溶液所需的质量、体积或浓度。
EnzymoPure™,His标签,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
L-阿拉伯糖异构酶,EC:5.3.1.4,来源于Bifidobacterium longum,催化L-arabinose可逆异构化为l -核酮糖,是磷酸戊糖或磷酸酮醇酶途径的一种组分,L-阿拉伯糖异构酶也被称为半乳糖异构酶,因为它们能够在体外将d-半乳糖异构为d-塔格糖。
反应式
L-arabinose——L-ribulose
使用方法
1. 按照反应体系加入底物10mM MnCl₂,可适当加入缓冲盐如:Tris-HCL,调节pH为6.8,加入适量L-阿拉伯糖异构酶 (根据反应需求,固体可加入适量纯水溶解后取用);
2. 混匀后,将样品置于60°C孵育1h。
【注】体系放大时候,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途。
L-Arabinose isomerase, EC: 5.3.1.4, derived from Bifidobacterium longum. It catalyzes the reversible isomerization of L-arabinose to L-ribulose and is a component of the pentose phosphate or phosphoketolase pathway. L-arabinose isomerase is also known as galactose isomerase because it can isomerize D-galactose to D-tagatose in vitro.
Reaction Equation
L-arabinose——L-ribulose
Instructions for Use
1. Add substrate and 10 mM MnCl₂ into the reaction system. Buffer salts such as Tris-HCl may be added as appropriate; adjust pH to 6.8. Add an appropriate amount of L-arabinose isomerase (for solid enzyme, dissolve with proper amount of pure water before use according to reaction requirements).
2. After mixing, incubate the sample at 60 °C for 1 h.
Note: When scaling up the system, incubation time or enzyme dosage shall be adjusted according to actual conditions. Low-speed shaking mixing is recommended.
Precautions
For your safety and health, please wear a lab coat and disposable gloves during operation. This product is for research use only.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 EnzymoPure™ 级别指南 → 查看 His Tag 级别指南 →