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| 配方(每升) | 含量 |
| 蛋白胨 | 10.0 g |
| 氯化钠 | 5.0g |
| 葡萄糖 | 1.0g |
| 酵母膏粉 | 5.0g |
| 琼脂 | 15.0g |
| 最终pH | 7.0±0.2 |
| 质控菌株及编号 | 指标 | 培养条件 | 质控评定标准 |
| 大肠埃希氏菌ATCC25922 | 生长率 | 36℃±1℃培养18~24h | PR≥0.7,灰白色菌落 |
| 大肠埃希氏菌ATCC8739 |
LB Agar is a growth media for the cultivation of a wide variety of microorganisms.
Used for general bacterial culture, especially for the preservation and culture of Escherichia coli in molecular biology experiments.
Testing principle
Peptone and yeast extract powder provide nitrogen sources, vitamins, and growth factors; sodium chloride maintains a balanced osmotic pressure; glucose provides a carbon source; and agar is the coagulant for the culture medium.
Ingredients of LB agar medium formula
| Formula (per liter) | Content |
Peptone |
10.0 g |
Sodium chloride |
5.0g |
Glucose |
1.0g |
Yeast extract powder |
5.0g |
Agar |
15.0g |
Final pH |
7.0±0.2 |
Instructions for use
Weigh 36g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, then aliquot into test tubes or conical flasks, sterilize at 121℃ for 15 minutes, and set aside for later use.
LB agar medium quality control [inoculation of the following quality control strains into the test medium]:
Quality control strain and number |
Indicator |
Culture conditions |
Quality control evaluation criteria |
Escherichia coli ATCC25922 |
Growth rate |
Cultured at 36℃±1℃ for 18~24h |
PR≥0.7, grayish-white colonies |
Escherichia coli ATCC8739 |