计算溶液所需的质量、体积或浓度。
Luxol 固蓝染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
髓鞘 (Myelin Sheath)是包裹在神经细胞轴突外面的一层膜，即髓鞘由髓鞘细胞和细胞膜组成，是神经膜细胞的质膜沿着轴索的轴心螺旋缠绕形成的多层脂双层结构,髓鞘上有郎飞氏结，可使神经冲动跳跃传递。髓鞘染色在病理诊断中有一定意义，髓鞘的病理变化分为早期、中期和晚期，在早期着色较深；病变中期阶段的髓鞘变性形成脂滴，可用脂质染色加以显示，后期彻底溃变并被吞噬细胞清除，故不再有髓鞘的阳性结果。
很多疾病都可以引起髓鞘的变化，Luxol Fast Blue染色可以显示病理情况下髓鞘是否完整、变性、坏死程度及修复情况，对神经组织的病理诊断和研究均有意义,例如神经纤维受损时，髓鞘可出现膨胀、曲折成球形、断裂或脱鞘完全消失等改变。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、蒸馏水、系列乙醇、二甲苯或脱蜡液 (D292661)
2、伊红染色液、0.1%碳酸锂水溶液、中性树胶
操作步骤(仅供参考)：
1、石蜡切片5-10μm，二甲苯或脱蜡液 (D292661)脱蜡，切片下行入95%乙醇稍洗。
2、加入Luxol Fast Blue染色液，室温染色12-20h。
3、加入95%乙醇洗去多余染色液，蒸馏水冲洗。
4、0.1%碳酸锂水溶液分色10-15s， 加入70%乙醇分色30s至灰白质清晰。
5、蒸馏水冲洗 (如果分色不足，可重复4-5步骤)。
6、伊红复染20s-90s，水洗。
7、常规脱水，二甲苯或脱蜡液 (D292661)透明，中性树胶封固。
染色结果：
髓鞘：蓝绿色
细胞质：红色
注意事项：
1、分化很关键，应严格控制分化时间，可在镜下观察分化程度。
2、固定液以10%的福尔马林为佳。
3、切片不宜太厚，应控制在5-10μm以内，否则易出现脱片或过染等现象。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Myelin Sheath is a membrane that wraps around the axon of a nerve cell. Specifically, it consists of myelin cells and cell membranes, and is a multi-layered lipid bilayer structure formed by the plasma membrane of neurolemmocytes spirally winding along the axis of the axon. There are nodes of Ranvier on the myelin sheath, which enable the saltatory conduction of nerve impulses. Myelin sheath staining has certain significance in pathological diagnosis. The pathological changes of the myelin sheath are divided into early, middle and late stages. In the early stage, the staining is relatively dark; in the middle stage of the lesion, the myelin sheath degenerates to form lipid droplets, which can be displayed by lipid staining; in the late stage, it completely degenerates and is cleared by phagocytes, so there is no longer a positive result for the myelin sheath.
Many diseases can cause changes in the myelin sheath. Luxol Fast Blue staining can show whether the myelin sheath is intact, the degree of degeneration and necrosis, and the repair status under pathological conditions, which is of significance for the pathological diagnosis and research of nerve tissue. For example, when nerve fibers are damaged, the myelin sheath may show changes such as swelling, twisting into a spherical shape, fragmentation, or complete disappearance of demyelination. This reagent is only used in the field of scientific research and is not suitable for clinical diagnosis or other purposes.
Self-Prepared Materials
1. Distilled water, a series of ethanol, xylene or dewaxing solution (D292661)
2. Eosin staining solution, 0.1% lithium carbonate aqueous solution, neutral balsam
Operating Steps (for reference only)
1. Paraffin sections (5-10 μm in thickness) are dewaxed with xylene or dewaxing solution (D292661), and then the sections are transferred downward into 95% ethanol for a brief rinse.
2. Add Luxol Fast Blue staining solution and stain at room temperature for 12-20 hours.
3. Rinse off the excess staining solution with 95% ethanol, then rinse with distilled water.
4. Differentiate with 0.1% lithium carbonate aqueous solution for 10-15 seconds, then differentiate with 70% ethanol for 30 seconds until the gray and white matter are clearly distinguishable.
5. Rinse with distilled water (if the differentiation is insufficient, steps 4-5 can be repeated).
6. Counterstain with eosin for 20 seconds to 90 seconds, then rinse with water.
7. Perform conventional dehydration, clear the sections with xylene or dewaxing solution (D292661), and mount the sections with neutral balsam.
Staining Results
Myelin sheath: Blue-green
Cytoplasm: Red
Precautions
1. Differentiation is crucial. The differentiation time should be strictly controlled, and the degree of differentiation can be observed under a microscope.
2. The preferred fixative is 10% formalin.
3. The sections should not be too thick and should be controlled within 5-10 μm; otherwise, phenomena such as section detachment or over-staining are likely to occur.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
规格或纯度
BioReagent,用于显微镜,生物染色剂
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Luxol Fast Blue Staining Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F1230638 |
分析证书 | L1507842 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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