鲁哥氏碘液也称 Lugol's碘液或鲁哥氏溶液或鲁哥试剂，是碘和碘化钾的水溶液，是 Lugol在1829年发明，并以其姓名命名，Lugol's碘液过去经常被用作消毒剂和杀菌剂，用于饮用水的应急消毒，实验室中用于检测淀粉等多种物质。
阿拉丁 Lugol's碘液采用经典配方, 其用途如下：检测叶片、食物等样品中的淀粉；细胞染色，使细胞核着色从而更易观察；Schiller Test在阴道镜中检查阴道和子宫颈是否癌变。正常阴道组织由于其高糖原质含量而被染成棕褐色，癌变的细胞则不会被染上颜色，与周围组织相比显现浅色；氧化性杀菌剂等，Lugol's碘液(5%)适合于作为媒染剂与结晶紫、沙黄等联合使用，对革兰氏细菌进行染色，其碘的含量为5%，经无菌处理，特别适合作为媒染剂对子宫颈进行涂抹染色，进而判断宫颈癌的可能。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
1、根据实验具体要求操作或参考如下步骤。
2、使用盐水棉球或干棉签轻轻插拭子宫颈，注意不应太用力，避免子宫颈上皮破损。
3、使用鲁哥氏染色液 (5%，无菌) 浸泡的棉球涂抹子宫颈，棉球应足够大，全部覆盖子宫颈；轻轻用棉球上下左右按压子宫颈 (包括子宫颈外口)，使Lugol's碘液充分渗透子宫颈上皮。一分钟后，用普通光源照明，直接观察子宫颈上皮对Lugol's碘液的反应。
4、根据未着色的程度、表面形态、边界状况、大小、与鳞柱交界的距离等，做出初步诊断。正常子宫颈上皮在涂碘后为赤褐色或黑色，未着色区或仅部分碘染为可疑病变区。CIN病变或浸润癌区域呈现厚重的芥末黄或桔黄色白斑。在阴道镜检查中应用碘溶液有助于识别醋酸方法遗漏的病变区域，并将更清晰地显示异常范围。该检测结果分为VILI阴性、VILI阳性、可疑癌，结果应记入临床检查表。
注意事项：
1、鲁哥氏染色液 (5%，无菌) 经无菌处理，注意避免污染。
2、该试剂仅用于科研用途，不可用于临床诊断。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Lugol’s Iodine Solution, also known as Lugol’s Iodine, Lugol’s Solution or Lugol’s Reagent, is an aqueous solution of iodine and potassium iodide. Invented by Lugol in 1829, it was named after its creator. In the past, Lugol’s Iodine Solution was often used as a disinfectant and bactericide for emergency disinfection of drinking water, and also for detecting starch and various other substances in laboratories.
Aladdin Lugol’s Iodine Solution adopts a classic formula and has the following applications: Detecting starch in samples such as leaves and food; Cell staining: it stains cell nuclei to facilitate observation; Schiller Test: used in colposcopy to screen for cancerous lesions of the vagina and cervix. Normal vaginal tissues are stained brown due to their high glycogen content, while cancerous cells remain unstained and appear pale in contrast to the surrounding tissues; Oxidizing bactericide, etc. The 5% Lugol’s Iodine Solution is suitable for use as a mordant combined with crystal violet, safranine and other dyes for Gram staining of bacteria. With an iodine content of 5% and subjected to sterile processing, it is particularly suitable for application as a mordant for smearing and staining the cervix to assess the possibility of cervical cancer. This reagent is for research use only and not suitable for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only):
1. Operate in accordance with the specific requirements of the experiment or refer to the following steps.
2. Gently swab the cervix with a normal saline-moistened cotton ball or a dry cotton swab. Do not apply excessive force to avoid damaging the cervical epithelium.
3. Smear the cervix with a cotton ball soaked in 5% sterile Lugol’s Iodine Stain. The cotton ball should be large enough to fully cover the cervix. Gently press the cervix (including the external cervical os) with the cotton ball in upward, downward, leftward and rightward directions to allow the Lugol’s Iodine Solution to fully penetrate the cervical epithelium. After one minute, illuminate the area with a regular light source and directly observe the reaction of the cervical epithelium to the Lugol’s Iodine Solution.
4. Make a preliminary diagnosis based on the degree of non-staining, surface morphology, boundary conditions, size, distance from the squamocolumnar junction, etc. Normal cervical epithelium turns reddish brown or black after iodine application; non-stained areas or partially iodine-stained areas are regarded as suspicious lesion areas. Regions with CIN (cervical intraepithelial neoplasia) lesions or invasive carcinoma present as thick mustard-yellow or orange-yellow leukoplakia. The application of iodine solution during colposcopy helps identify lesion areas missed by the acetic acid method and can more clearly delineate the scope of abnormalities. The test results are classified as VILI (visual inspection with Lugol’s iodine) negative, VILI positive, or suspected cancer, and the results should be recorded in the clinical examination form.
Precautions:
1. The 5% sterile Lugol’s Iodine Stain has undergone sterile processing; please take precautions to avoid contamination.
2. This reagent is for research use only and shall not be used for clinical diagnosis.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
ZJ26F1039669
危险品化学品经营许可证（带存储）