计算溶液所需的质量、体积或浓度。
脂褐素染色液 (Long Ziehl-Neelsen法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
脂褐素是具有颗粒状的褐黄色色素，由含有脂肪的残存物和溶酶体消化物组成，被认为是由脂质和脂蛋白氧化产生的。脂褐素氧化过程是缓慢的、逐步发生的，因此色素会呈现出不同的染色反应、不同的颜色，形状和大小也变化不一，脂褐素可见于肝脏、肾脏、心肌、肾上腺、神经细胞与神经节细胞等，多分布在细胞核周围。
由于脂褐素是由脂质和脂蛋白缓慢氧化逐步形成的，色素所处的氧化程度不同，因此应用技术证实时，组织化学反应会有所不同，因此建议应用多种不同的技术来验证色素是脂褐质。常用的方法有PAS法、Schmorl高铁-铁氰化物还原法、Long Ziehl-Neelsen法、Gomori醛复红法、Masson-Fontana银法等。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、载玻片、恒温箱或水浴锅
2、梯度乙醇、二甲苯或环保脱蜡透明液、中性树胶
操作步骤(仅供参考)：
1、组织固定，常规脱水包埋。
2、常规二甲苯或脱蜡透明液脱蜡至水。
3、组织切片裱贴于载玻片上，入蒸馏水轻轻清洗。
4、入复红染色液加盖浸染，60℃水浴3h或室温过夜，蒸馏水洗净。
5、入Long酸性分化液中分化，直至背景染色被去除。蒸馏水洗净。
6、入亚甲蓝染色液复染胞核1min。
7、入乙酸溶液轻轻清洗。
8、常规脱水，常规二甲苯或脱蜡透明液透明，中性树胶封片。
染色结果：
脂褐素、蜡样物质: 紫红色
细胞核: 蓝色
背景: 淡红紫色或淡蓝色
注意事项：
1、恒温控制的水浴条件下进行染色，可以得到更可靠的结果。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用，以防影响后续实验效果。
Lipofuscin is a granular yellowish-brown pigment composed of lipid-containing residues and lysosomal digests, which is considered to be produced by the oxidation of lipids and lipoproteins. The oxidation process of lipofuscin is slow and gradual, resulting in the pigment exhibiting diverse staining reactions and colors, as well as variable shapes and sizes. Lipofuscin can be found in the liver, kidneys, myocardium, adrenal glands, neurons and ganglion cells, and is mostly distributed around the cell nucleus.
Since lipofuscin is gradually formed by the slow oxidation of lipids and lipoproteins, the oxidation degree of the pigment varies. Therefore, the histochemical reactions will differ when verified by technical methods. It is recommended to use a variety of different techniques to confirm that the pigment is lipofuscin. Common methods include the PAS method, Schmorl's ferric-ferricyanide reduction method, Long Ziehl-Neelsen method, Gomori's aldehyde-fuchsin method, Masson-Fontana silver method, etc. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by User
1. Glass slides, incubator or water bath
2. Gradient ethanol, xylene or environment-friendly dewaxing clearing solution, neutral mounting medium
Procedure (for reference only)
1. Fix the tissue, and perform routine dehydration and embedding.
2. Dewax the tissue sections to water using routine xylene or dewaxing clearing solution.
3. Mount the tissue sections on glass slides and rinse gently with distilled water.
4. Cover the sections with Fuchsin Staining Solution, incubate in a water bath at 60℃ for 3 hours or at room temperature overnight, then rinse thoroughly with distilled water.
5. Differentiate the sections in Long Acid Differentiation Solution until the background staining is removed. Rinse thoroughly with distilled water.
6. Counterstain the cell nuclei with Methylene Blue Staining Solution for 1 minute.
7. Rinse the sections gently with acetic acid solution.
8. Perform routine dehydration, clear the sections with routine xylene or dewaxing clearing solution, and mount with neutral mounting medium.
Staining Results
Lipofuscin and waxy substances: Fuchsia
Cell nuclei: Blue
Background: Pale reddish-purple or pale blue
Precautions
1. More reliable results can be obtained when staining is performed under temperature-controlled water bath conditions.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
脂褐素染色液
英文别名
Lipofuscin Staining Solution
规格或纯度
BioReagent,用于显微镜,生物染色剂
英文名称
Lipofuscin Staining Solution (Long Ziehl-Neelsen Method)
应用
细胞染色
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
各组分在相应的储存条件下保质期1年。
组分与储存
| L1508454 | Component | 4×50 mL | Storage |
| L1508454A | 复红染色液 | 50 mL | RT. Store in the dark. |
| L1508454B | Long酸性分化液 | 50 mL | RT |
| L1508454C | 亚甲蓝染色液 | 50 mL | RT. Store in the dark. |
| L1508454D | 乙醇溶液 | 50 mL | RT |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332734 |
分析证书 | L1508454 |
技术文档和文章
溶液计算器
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