病原线虫在病组织中可通过挑针直接挑取、漏斗分离或将病组织捣碎分离等方法取得活线虫虫体，为了识别常见线虫，对其形态和结构做一般性观察，可以用活体线虫制作临时玻片，为了进一步仔细观察、认识其分类学特征，需将活体线虫杀死和固定，有时还需染色，制成永久性玻片标本，如需观察线虫与寄主组织之间的相互关系，还需要制作寄主和线虫组织的石蜡切片。
阿拉丁线虫活体染色液 (碘法) 主要由碘、碘化钾等组成，用于含线虫的植物组织样本的活体染色观察，特别适用于根内线虫材料，其原理是碘液浸入到根组织内，使线虫着色而植物组织无色，便于区分组织和线虫。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
蒸馏水、载玻片、显微镜、镊子、处理针、甘油、封片剂等
操作步骤(仅供参考)：
1、用蒸馏水清洗植物的病理状态的根部，吸水纸吸干水分。
2、用镊子将病根浸入到碘液内染色10~15min。
3、镊子取出组织用蒸馏水清洗。
4、放置于载玻片上，用处理针找到线虫，观察组织和线虫的染色情况。
5、如果需要可以压片观察线虫内部器官的染色情况。
6、用Arfagal封固液制作临时装片或永久装片。
染色结果：
注意事项：
1、玻片应洁净，无油污。
2、线虫的染色标本置于无水甘油中可保存2～5个月，之后会逐渐褪色。
3、一般情况下线虫被染成棕黄色-黑色，但并不是所有特征都能染出来，即染色结果不一定完全符合上述表格。
4、固定时不宜用高热火焰固定。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Live nematode specimens can be obtained from infected tissues via direct picking with a needle, Baermann funnel extraction, or grinding the infected tissues followed by separation. For the identification of common nematode species, general observations of their morphology and structure can be conducted by preparing temporary slides using live nematodes. To further examine and characterize their taxonomic features in detail, live nematodes need to be killed, fixed, and sometimes stained to prepare permanent slide specimens. If the goal is to observe the interaction between nematodes and host tissues, paraffin sections of both host and nematode tissues are required.
Aladdin Nematode Vital Stain Solution (Iodine Method) is mainly composed of iodine, potassium iodide, and other ingredients. It is designed for the vital staining and observation of plant tissue samples containing nematodes, and is particularly suitable for root-inhabiting nematode materials. Its working principle relies on the penetration of iodine solution into root tissues, which stains the nematodes while leaving the plant tissues unstained, thus facilitating the distinction between the two. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.
Self-prepared Materials:
Distilled water, glass slides, microscopes, tweezers, dissecting needles, glycerol, mounting media, etc.
Operating Procedures (For Reference Only):
1. Rinse the diseased roots of plants with distilled water and blot the water dry with absorbent paper.
2. Immerse the diseased roots in iodine solution with tweezers and stain for 10–15 minutes.
3. Take out the tissues with tweezers and rinse them with distilled water.
4. Place the tissues on a glass slide, locate the nematodes with a dissecting needle, and observe the staining status of the tissues and nematodes.
5. If necessary, squash the preparation to observe the staining of the internal organs of the nematodes.
6. Prepare temporary or permanent mounts using Arfagal mounting medium.
Staining Results:
Nematodes
| Brownish yellow to black
|
Pathological samples
| Colorless
|
Preautions:
1. Glass slides shall be clean and free of oil stains.
2. Stained nematode specimens can be preserved in anhydrous glycerol for 2–5 months, after which they will gradually fade.
3. Under normal circumstances, nematodes will be stained brownish yellow to black, but not all morphological characteristics can be visualized by staining. That is to say, the staining results may not be completely consistent with the table above.
4. High-temperature flame fixation should be avoided during the fixation process.
5. For your safety and health, please wear a lab coat and disposable gloves when performing the operation.
6. Please use the reagent as soon as possible after opening to prevent affecting the subsequent experimental results.
ZJ26F0232072
危险品化学品经营许可证（带存储）