计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
木质素是三种苯丙烷单元通过醚键和碳碳键相互连接形成的具有三维网状结构的生物高分子,存在于木质组织中,主要作用是通过形成交织网来硬化细胞壁,为次生壁主要成分。木质素主要位于纤维素纤维之间,起抗压作用。在木本植物中木质素占25%,是世界上第二位丰富的有机物,由于自然界中木质素与纤维素、半纤维素等往往相互连接,形成木质素-碳水化合物复合体(Lignin-Carbohydrate Complex),故目前没有办法分离得到结构完全不受破坏的原本木质素。
木质素染色液(甲基红法)利用甲基红将木质素染色,染色后木质化的细胞壁呈黄色,该染色法是简便的检测植物细胞壁的木质素的显微化学法,但该染色会随着时间逐渐褪色,因此不适用于作永久制片。该试剂仅用于科研领域,不用于临床诊断或其他用途。
自备材料
载玻片、盖玻片、显微镜
操作步骤(仅供参考)
1、将切好的切片材料置于载玻片上,滴加50-100μl甲基红染色液,稍放置浸透材料。
2、盖上盖玻片,显微镜下观察。
染色结果
木质化的细胞壁呈黄色。
注意事项
1、木质化程度越强,颜色越深。
2、一般建议使用徒手切片或者冰冻切片,保持样本内容物的完整性。
3、尽量避免使用石蜡切片,可能因石蜡包埋过程复杂导致木质素丢失。
4、染色会随着时间逐渐褪色,因此不适用于作永久制片。
5、试剂开封后请尽快使用,以防影响后续实验效果。
Lignin is a biopolymer with a three-dimensional network structure formed by the interconnection of three phenylpropane units via ether and carbon-carbon bonds. It is present in lignified tissues and primarily functions to harden cell walls by forming an interwoven network, serving as a major component of the secondary wall. Lignin is mainly located between cellulose fibers, providing compressive strength. In woody plants, lignin constitutes about 25% of the biomass and is the second most abundant organic compound in the world. In nature, lignin is often linked with cellulose and hemicellulose, forming a Lignin-Carbohydrate Complex (LCC). Consequently, it is currently not possible to isolate native lignin with its structure completely intact.
Principle
The Lignin Staining Solution (Methyl Red Method) utilizes methyl red to stain lignin. After staining, lignified cell walls appear yellow. This staining method is a simple microchemical technique for detecting lignin in plant cell walls. However, the stain fades over time and is therefore not suitable for preparing permanent slides. This reagent is for research use only, not for clinical diagnosis or other purposes.
Reagents, consumables and Equipments not provided
Glass slides, Cover slips, Microscope
Procedure (For Reference Only)
1. Place the prepared sectioned material on a glass slide. Add 50-100 µL of Methyl Red Staining Solution and allow it to sit briefly to permeate the material.
2. Place a cover slip on top and observe under a microscope.
Staining Result
Lignified cell walls appear yellow.
Precautions
1. The greater the degree of lignification, the deeper the color.
2. It is generally recommended to use free-hand sections or frozen sections to preserve the integrity of the sample contents.
3. The use of paraffin sections should be avoided as much as possible, as the complex paraffin embedding process may lead to lignin loss.
4. The stain fades over time and is therefore not suitable for preparing permanent slides.
5. After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1509205 |
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