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BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
木质素是三种苯丙烷单元通过醚键和碳碳键相互连接形成的具有三维网状结构的生物高分子,存在于木质组织中,主要作用是通过形成交织网来硬化细胞壁,为次生壁主要成分。木质素主要位于纤维素纤维之间,起抗压作用。在木本植物中木质素占25%,是世界上第二位丰富的有机物,由于自然界中木质素与纤维素、半纤维素等往往相互连接,形成木质素-碳水化合物复合体(Lignin-Carbohydrate Complex),故目前没有办法分离得到结构完全不受破坏的原本木质素。
木质素染色液(Pianeze法)又称皮阿尼兹染色液、混合染料染色液,由孔雀石绿、酸性品红、去离子水等制成,可区分纤维素细胞壁和木质化细胞壁,染色后木质化细胞壁呈青色,纤维素细胞壁呈红色,木栓化细胞壁不上色。其他常用的方法有间苯三酚法(Weisner染色)、酸化苯胺法、番红染色法和Maule染色法等,其中,间苯三酚法是最简便、最常用的检测植物细胞壁木质素的显微化学法。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
| L1509212 | Component | 50 mL | Storage |
| L1509212A | Pianeze染色液 | 50 mL | RT. Store in the dark. |
| L1509212B | 甘油封片剂 | 10 mL | RT. |
自备材料
载玻片、盖玻片、显微镜、去离子水或蒸馏水
操作步骤(仅供参考)
1、将切好的切片放入Pianeze染色液中密封避光浸染30分钟~3小时。
2、蒸馏水漂洗1~3分钟。
3、滴加一滴甘油封片剂封片,显微镜下可见光观察并拍照。
染色结果
木质化细胞壁呈青色,纤维素细胞壁呈红色,木栓化细胞壁不上色。
注意事项
1、木质化程度越强,颜色越深。
2、一般建议使用徒手切片或者冰冻切片,保持样本内容物的完整性。
3、尽量避免使用石蜡切片,可能因石蜡包埋过程复杂导致木质素丢失。
4、染色会随着时间逐渐褪色,因此不适用于作永久制片。
5、Pianeze染色液有挥发性,用后请及时拧紧瓶盖。
6、试剂开封后请尽快使用,以防影响后续实验效果。
7、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Lignin is a biopolymer with a three-dimensional network structure formed by the interconnection of three phenylpropane units via ether and carbon-carbon bonds. It is present in lignified tissues and primarily functions to harden cell walls by forming an interwoven network, serving as a major component of the secondary wall. Lignin is mainly located between cellulose fibers, providing compressive strength. In woody plants, lignin constitutes about 25% of the biomass and is the second most abundant organic compound in the world. In nature, lignin is often linked with cellulose and hemicellulose, forming a Lignin-Carbohydrate Complex (LCC). Consequently, it is currently not possible to isolate native lignin with its structure completely intact.
Principle:
The Lignin Staining Solution (Pianeze Method), also known as Pianeze stain or mixed dye stain, is prepared from malachite green, acid fuchsin, deionized water, etc. It can differentiate between cellulose cell walls and lignified cell walls. After staining, lignified cell walls appear cyan (bluish-green), cellulose cell walls appear red, and suberized cell walls remain unstained. Other commonly used methods include the phloroglucinol method (Weisner staining), acidified aniline method, safranin staining, and Mäule staining. Among these, the phloroglucinol method is the simplest and most frequently used microchemical technique for detecting lignin in plant cell walls. This reagent is for research use only, not suitable for clinical diagnosis or other purposes.
| L1509212 | Component | 50 mL | Storage |
| L1509212A | Pianeze Staining Solution | 50 mL | RT. Store in the dark. |
| L1509212B | Glycerol Mounting Medium | 10 mL | RT. |
Reagents, consumables and Equipments not provided
Glass slides, Cover slips, Microscope, Deionized water or Distilled water.
Procedure (For Reference Only)
Place the prepared sections into the Pianeze Staining Solution. Seal the container and stain protected from light for 30 minutes to 3 hours.
Rinse with distilled water for 1 to 3 minutes.
Add one drop of Glycerol Mounting Medium to mount the section. Observe and photograph under a microscope using visible light.
Staining Result
Lignified cell walls appear cyan (bluish-green), cellulose cell walls appear red, and suberized cell walls remain unstained.
Precautions
The greater the degree of lignification, the deeper the color.
It is generally recommended to use free-hand sections or frozen sections to preserve the integrity of the sample contents.
The use of paraffin sections should be avoided as much as possible, as the complex paraffin embedding process may lead to lignin loss.
The stain fades over time and is therefore not suitable for preparing permanent slides.
Pianeze Staining Solution is volatile. Tighten the bottle cap immediately after use.
After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.
For your safety and health, please wear a lab coat and disposable gloves during operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1509212 |
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