储存缓冲液：Lyophilized from bulk protein in a 0.22 μm filtered solution of 0.01 M PB, 0.15 M NaCl, 3% trehalose, pH=7.4.
复溶：Reconstitute in ultrapure water.
阿拉丁生产的DBCO修饰的藻红蛋白 (DBCO-PE)是一种即用型的点击化学偶联试剂，采用高纯度藻红蛋白与DBCO-PEG5-NHS ester共价修饰而成。该产品可与叠氮修饰的蛋白、抗体、寡核苷酸、聚合物或其它分子在温和条件下实现快速、高效的SPAAC 反应。
藻红蛋白（PE，R-PE）是一种发红光的荧光蛋白-发色团复合物，可被488 nm蓝光、532 nm绿光或561 nm黄绿光激发，激发效率依次递增，并可使用586/14 nm带通滤光片进行检测。PE的激发峰位于565 nm，发射峰位于578 nm。R-PE是一种大分子（约240 kDa），用于基于荧光的检测，主要应用于流式细胞术、微阵列分析、ELISA以及其他需要高灵敏度但对光稳定性要求不高的检测场景。而DBCO（二苯基环辛炔）是一种环炔烃，能够与叠氮化物在温和条件下发生高效的、无铜催化的点击化学反应，这种反应具有高选择性、快速动力学和良好的生物相容性，适用于活细胞和体内环境。聚乙二醇（PEG）链的引入则增强了试剂的溶解性、稳定性和生物相容性，减少非特异性结合，延长循环半衰期。DBCO-PE 融合了藻红蛋白与DBCO基团的优势，能够与叠氮修饰的蛋白质、多肽和寡核苷酸实现高效、无铜的偶联，适用于流式细胞术、成像及检测方法开发。
该DBCO-PE产品通过尺寸排阻色谱法进行纯化，去除了未偶联的分子，确保其最佳染色效果。
使用建议
储存条件：冻干粉在2-8℃干燥、避光长期保存，请勿冷冻；复溶后溶液建议4℃保存不超过1周。藻胆蛋白对冻融循环敏感。避免受热和光照。
无铜点击反应：
1. 以叠氮修饰的抗体标记为例：DBCO-PE推荐使用浓度为5 mg/mL，标记质量比为IgG:PE = 1:3。将DBCO-PE与叠氮修饰的蛋白或抗体混合。。
2. 在室温下孵育 2~4 小时，或在 4°C 下孵育过夜。
3. 根据您的实验应用，可直接使用偶联后的蛋白，或通过尺寸排阻色谱柱对偶联产物进行纯化。
DBCO-modified Phycoerythrin (DBCO-PE) produced by Aladdin is a ready-to-use click chemistry conjugation reagent, prepared by covalently modifying high-purity R-Phycoerythrin with DBCO-PEG5-NHS ester. This product enables rapid and efficient copper-free SPAAC (strain-promoted azide-alkyne cycloaddition) with azide-modified proteins, antibodies, oligonucleotides, polymers, or other molecules under mild conditions.
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency, and detected using a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. R-PE is a large molecule (~240 kDa) used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. DBCO (dibenzocyclooctyne) is a cyclooctyne that undergoes efficient, copper-free click chemistry with azides under mild conditions. This reaction features high selectivity, fast kinetics, and excellent biocompatibility, making it suitable for live-cell and in vivo applications. The introduction of a polyethylene glycol (PEG) spacer enhances the reagent’s solubility, stability, and biocompatibility, reduces non-specific binding, and prolongs circulation half-life. DBCO-PE combines the advantages of phycoerythrin and DBCO groups, enabling efficient, copper-free conjugation to azide-modified proteins, peptides, and oligonucleotides, and is well-suited for flow cytometry, imaging, and assay development.
This DBCO-PE product is purified by size exclusion chromatography to remove unconjugated molecules, ensuring optimal staining performance.
Usage Suggestion
Storage conditions: The lyophilized powder should be stored dry and protected from light at 2–8°C for long-term preservation. Do not freeze. After reconstitution, the solution is recommended to be stored at 4°C for no more than 1 week. Phycobiliproteins are sensitive to freeze-thaw cycles. Avoid exposure to heat and light.
Copper-Free click reaction:
1. For azide-modified antibody labeling as an example: DBCO-PE is recommended at a concentration of 5 mg/mL, with a labeling mass ratio of IgG:PE = 1:3. Mix DBCO-PE with the azide-modified protein or antibody.
2. Incubated for 2-4 hours at room temperature or overnight at 4°C.
3. Depending on your application, you can use the conjugated protein immediately or purify the conjugated protein by size exclusion chromatography columns and resins.
ZJ26F0535837
危险品化学品经营许可证（带存储）