阿拉丁生产的DBCO修饰的别藻蓝蛋白（DBCO-APC） 是一种即用型的点击化学偶联试剂，采用高纯度别藻蓝蛋白与DBCO-PEG5-NHS酯共价修饰而成。该产品可与叠氮修饰的蛋白、抗体、寡核苷酸、聚合物或其他分子在温和条件下实现快速、高效的无铜SPAAC（张力促进的叠氮-炔环加成）反应。
别藻蓝蛋白（APC）是一种来源于蓝藻和红藻的荧光蛋白，可作为高效的供体荧光染料，用于构建能被633-640 nm激光激发的串联染料。APC的激发峰位于650 nm，发射峰位于660 nm。APC是一种较大的化学交联分子（约104 kDa），用于基于荧光的检测，主要应用于流式细胞术、微阵列分析、ELISA以及其他需要高灵敏度但对光稳定性要求不高的检测场景。DBCO（二苯基环辛炔）是一种环炔烃，能够在温和条件下与叠氮化物发生高效、无铜催化的点击化学反应，该反应具有高选择性、快速动力学和良好的生物相容性，适用于活细胞及体内应用。聚乙二醇（PEG）链的引入增强了试剂的溶解性、稳定性和生物相容性，减少了非特异性结合，并延长了循环半衰期。DBCO-APC融合了别藻蓝蛋白与DBCO基团的优势，能够与叠氮修饰的蛋白、抗体、多肽和寡核苷酸实现高效、无铜的偶联，非常适用于流式细胞术、成像及检测方法开发。
该DBCO-APC产品通过尺寸排阻色谱法进行纯化，去除了未偶联的分子，确保最佳的染色性能。
使用建议
储存条件： 冻干粉应于2-8°C条件下干燥、避光长期保存。请勿冷冻。复溶后的溶液建议在4°C保存不超过1周。藻胆蛋白对冻融循环敏感。避免受热和光照。
无铜点击反应：
1. 以叠氮修饰的抗体标记为例：DBCO-APC推荐使用浓度为5 mg/mL，标记质量比为IgG:APC = 1:2。将DBCO-APC与叠氮修饰的蛋白或抗体混合。
2. 在室温下孵育2-4小时，或在4°C下孵育过夜。
3. 根据您的实验应用，偶联后的蛋白可直接使用，或通过尺寸排阻色谱法进行纯化。
DBCO-modified Allophycocyanin (DBCO-APC) produced by Aladdin is a ready-to-use click chemistry conjugation reagent, prepared by covalently modifying high-purity Allophycocyanin with DBCO-PEG5-NHS ester. This product enables rapid and efficient copper-free SPAAC (strain-promoted azide-alkyne cycloaddition) with azide-modified proteins, antibodies, oligonucleotides, polymers, or other molecules under mild conditions.
Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm. APC is a large chemically-crosslinked molecule (~104KD) used in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. DBCO (dibenzocyclooctyne) is a cyclooctyne that undergoes efficient, copper-free click chemistry with azides under mild conditions. This reaction features high selectivity, fast kinetics, and excellent biocompatibility, making it suitable for live-cell and in vivo applications. The introduction of a polyethylene glycol (PEG) spacer enhances the reagent’s solubility, stability, and biocompatibility, reduces non-specific binding, and prolongs circulation half-life. DBCO-APC combines the advantages of Allophycocyanin and DBCO groups, enabling efficient, copper-free conjugation to azide-modified proteins, peptides, and oligonucleotides, and is well-suited for flow cytometry, imaging, and assay development.
This DBCO-APC product is purified by size exclusion chromatography to remove unconjugated molecules, ensuring optimal staining performance.
Usage Suggestion
Storage conditions: The lyophilized powder should be stored dry and protected from light at 2–8°C for long-term preservation. Do not freeze. After reconstitution, the solution is recommended to be stored at 4°C for no more than 1 week. Phycobiliproteins are sensitive to freeze-thaw cycles. Avoid exposure to heat and light.
Copper-Free Click Reaction:
1. For azide-modified antibody labeling as an example: DBCO-APC is recommended at a concentration of 5 mg/mL, with a labeling mass ratio of IgG:APC = 1:2. Mix DBCO-APC with the azide-modified protein or antibody.
2. Incubate for 2–4 hours at room temperature, or overnight at 4°C.
3. Depending on your application, the conjugated protein can be used immediately or purified by size exclusion chromatography.
ZJ26F0535839
危险品化学品经营许可证（带存储）