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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脂质过氧化物（Lipid hydroperoxide，LPO）是不饱和脂肪酸链经自由基或活性氧作用后产生的过氧化物。病理情况下，脂质过氧化反应增强可导致原本低含量的 LPO 升高。LPO 含量升高会对细胞的结构和功能造成损伤，LPO 含量与机体免疫系统和衰老密切相关。本试剂盒检测原理是 LPO在酸性条件下加热产生丙二醛（MDA），MDA 与硫代巴比妥酸（Thiobarbituricacid，TBA）缩合，生成棕红色物质三甲川（3,5,5-三甲基恶唑-2,4-二酮），其最大吸收波长在532 nm，进行比色后可估测样本中 LPO 的含量。
Lipid hydroperoxides (LPO) are peroxides generated from unsaturated fatty acid chains under the action of free radicals or reactive oxygen species. Under pathological conditions, enhanced lipid peroxidation can lead to elevated levels of LPO, which are normally present at low concentrations. Increased LPO content can cause damage to cell structure and function, and it is closely associated with the body's immune system and aging. The detection principle of this kit is as follows: LPO produces malondialdehyde (MDA) upon heating under acidic conditions. MDA then condenses with thiobarbituric acid (TBA) to form a brown-red-colored product, trimethine (3,5,5-trimethyloxazole-2,4-dione), which exhibits a maximum absorption wavelength at 532 nm. Colorimetric measurement at this wavelength allows for the estimation of LPO content in the sample.
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