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脂质过氧化是动植物体内活性氧(ROS)氧化生物膜的过程，脂质过氧化物（LPO）是脂质过氧化过程的产物，即ROS与生物膜的磷脂、酶和膜受体相关的多不饱和脂肪酸的侧链及核酸等大分子物质起脂质过氧化反应形成LPO，使细胞膜的流动性和通透性发生改变,最终导致细胞结构和功能的改变。因此，LPO常被作为机体氧化应激的一种指标，与某些疾病的病理过程，如肿瘤、化学中毒、感染、炎 症、自身免疫疾病、动脉粥样硬化（AS）及心脑血管疾病，以及衰老等生理过程密切相关。 测定原理：LPO在酸性条件下加热，产生小分子终产物MDA，MDA与硫代巴比妥酸(thiobarbituric acid，TBA)缩合，生成红色产物，在535nm有最大吸收峰。
Lipid peroxidation is the process in which reactive oxygen species (ROS) oxidize biomembranes in animals and plants. Lipid peroxides (LPO) are the products of this process. Specifically, ROS react with macromolecules such as polyunsaturated fatty acid side chains of phospholipids, enzymes, and membrane receptors within the biomembrane, as well as nucleic acids, initiating the lipid peroxidation reaction to form LPO. This alters the fluidity and permeability of the cell membrane, ultimately leading to changes in cellular structure and function. Consequently, LPO is often used as an indicator of oxidative stress in the body and is closely associated with the pathological processes of certain diseases (such as tumors, chemical poisoning, infections, inflammation, autoimmune diseases, atherosclerosis, cardiovascular and cerebrovascular diseases) as well as physiological processes like aging. Assay Principle: Under acidic conditions and heating, LPO decomposes to yield small molecular end-products, including malondialdehyde (MDA). MDA then condenses with thiobarbituric acid (TBA) to form a red adduct, which exhibits a maximum absorption peak at 535 nm.
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