水凝胶是一类极为亲水的三维网络结构凝胶,由于其优秀的生物相容性,已成为3D生物打印有前途的生物墨水。然而,与水结合时,水凝胶往往表现出高度溶胀。溶胀会降低水凝胶的力学性能。而且,不受控制的溶胀使其植入体内时还可能损伤周围组织。此外,溶胀还会影响打印结构的精度。
低溶胀3D打印墨水是一款经无菌处理的,适用于DLP和LCD打印的即用型生物墨水。该产品通过引入有一定疏水性的高分子材料来控制水凝胶的溶胀度,从而保证打印精度。
产品规格
| 产品货号 | 外观 | 规格 | 备注 |
L1518387
| 黄色透明溶液 | 5 mL/支、10 mL/支 | 避光保存 |
产品包装
| 包装清单 | 备注 |
| 预灌封注射器 (内含墨水)、推杆、说明书 | 使用前将推杆与胶塞旋紧 |
性能参数
| 测试项目 | 性能参数 | 测试项目 | 性能参数 |
| 拉伸模量 | 1.0~1.5 MPa | 断裂延伸率 | 75~110% |
| 拉伸强度 | 2.2~3.0 MPa | 压缩强度 | 20~27 MPa |
| 含水量 | ≈74% | 溶胀度 | 12~20% |
| 溶液粘度(25℃) | 180~220 mpa.s | 溶液粘度(37℃) | 150~180 mpa.s |
| 细胞相容性 | 无潜在细胞毒性 | 打印精度 | 10-50 μm |
应用领域
1、高强度、高弹性植入器件 (如假体、气管等)
2、高精密、复杂水凝胶器件 (如支架、血管等)
3、多尺度微流控通道及类器官芯片
使用建议
1、本产品可与药物或因子等混合使用
① 配制药物或因子溶液,过膜除菌 (0.22 μm),并将其移入空的鲁尔接口注射器内。
② 将上述注射器与本产品通过鲁尔锁适配器连接,连接前小心地将鲁尔锁适配器预先填充,以免引入气泡。轻轻地来回推动,至墨水与药物或因子溶液完全混合均匀。
③ 如果单次使用量低于产品装样量,可预先将所需量墨水溶液通过鲁尔锁适配器转移至空的鲁尔接口注射器内。
④ 建议将十份墨水溶液与一份药物或因子等溶液混合,以免产品浓度过低影响固化后凝胶性能。
2、打印参数如下表所示
| 项目 | 参数 | 项目 | 参数 |
| 辐照波长 | 365 nm~405 nm | 推荐辐照波长 | 385 nm |
| 辐照强度 | ≥30 mW/cm² | 曝光时间 | 30~45 s |
| 打印层高 | 50-100 μm |
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3、打印后使用磷酸盐缓冲液或生理盐水对打印模型进行冲洗,将残余胶液冲洗干净,并对打印模型进行后固化 (推荐:385nm,≥50 mW/cm²,各方向30 s),至模型完全固化。
4、取完全固化后的打印模型置于磷酸盐缓冲液或生理盐水中反复浸泡3次,每次30 min,除去残留的光引发剂及光吸收剂小分子。注:与药物或因子等混合使用时,酌情处理。
注意事项
1、产品经无菌处理,取用时注意无菌操作,避免微生物污染。
2、使用前,产品注射器外壁可用酒精擦拭消毒。
3、产品需严格避光保存。
Product Introduction
Hydrogels are highly hydrophilic three-dimensional network-structured gels. Owing to their excellent biocompatibility, they have become promising bioinks for 3D bioprinting. However, hydrogels usually exhibit high swelling upon contact with water. Swelling degrades the mechanical properties of hydrogels. Moreover, uncontrolled swelling may damage the surrounding tissues when implanted in vivo. In addition, swelling compromises the precision of printed structures.
Low-swelling 3D printing ink is a sterile, ready-to-use bioink suitable for DLP and LCD printing. This product controls the swelling degree of hydrogels by introducing hydrophobic polymers, thereby ensuring printing accuracy.
Product Specifications
Product Code
| Appearance
| Specification
| Note
|
L1518387
| Yellow transparent solution
| 5 mL/vial、10 mL/vial | Store in the dark |
Product Packaging
| Package List | Note |
| Prefilled syringe (with ink), plunger, instruction manual | Tighten the plunger and rubber stopper before use |
Performance Parameters
| Test Item | Performance Parameter | Test Item | Performance Parameter |
| Tensile Modulus | 1.0~1.5 MPa | Elongation at Break | 75~110% |
| Tensile Strength | 2.2~3.0 MPa | Compressive Strength | 20~27 MPa |
| Water Content | ≈74% | Swelling Degree | 12~20% |
| Viscosity (25°C) | 180~220 mPa·s | Viscosity (37°C) | 150~180 mPa·s |
| Cytocompatibility | No potential cytotoxicity | Printing Accuracy | 10~50 μm |
Applications
1. High-strength, high-elasticity implantable devices (e.g., prostheses, trachea, etc.)
2. High-precision, complex hydrogel devices (e.g., scaffolds, blood vessels, etc.)
3. Multiscale microfluidic channels and organoid chips
Usage Instructions
1. This product can be mixed with drugs, factors or other bioactive substances.
① Prepare a solution of drugs or factors, filter-sterilize (0.22 μm), and transfer it into an empty luer-lock syringe.
② Connect the above syringe with the product syringe using a luer-lock adapter. Carefully pre-fill the luer-lock adapter before connection to avoid introducing air bubbles. Gently push back and forth until the ink is completely and uniformly mixed with the drug/factor solution.
③ If the single-use volume is less than the product loading volume, the required amount of ink solution can be pre-transferred into an empty luer-lock syringe via a luer-lock adapter.
④ It is recommended to mix 10 parts of ink solution with 1 part of drug/factor solution to avoid excessively low concentration, which may affect the gel performance after curing.
2. Printing parameters are shown in the table below
| Item | Parameter | Item | Parameter | | Irradiation Wavelength | 365 nm~405 nm | Recommended Irradiation Wavelength | 385 nm | | Irradiation Intensity | ≥ 30 mW/cm² | Exposure Time | 30~45 s | | Printing Layer Height | 50~100 μm | |
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3. After printing, rinse the printed model with phosphate-buffered saline (PBS) or physiological saline to remove residual ink. Post-cure the model (recommended: 385 nm, ≥ 50 mW/cm², 30 s on each side) until fully cured.
4. Immerse the fully cured model in PBS or physiological saline repeatedly 3 times, 30 min each time, to remove residual photoinitiator and light absorber small molecules.
Note: When used in combination with drugs or factors, handle appropriately.
Precautions
1. The product is sterilized. Maintain aseptic operation during use to avoid microbial contamination.
2. Before use, the outer surface of the product syringe can be wiped and disinfected with alcohol.
3. The product must be stored strictly away from light.