计算溶液所需的质量、体积或浓度。
PRP低析出分离胶
有货
为什么选择此级别
BioReagent,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本品为低析出型PRP分离胶，专门用于分离制备富含生长因子的富血小板血浆（PRP），可有效保护血浆中的生长因子活性。本品可与抗凝剂配合制成PRP真空采血管，广泛应用于临床治疗、组织修复再生及美容整形等领域，为PRP制备提供高效、洁净、稳定的分离保障。
| 主要成分 | 高分子聚合物、二氧化硅等 |
| 性状 | 本品为黄色透明胶体，具有良好的触变性和隔离效果，呈生理惰性，不与血液成分、抗凝剂发生反应，不干扰PRP中生长因子活性。 |
| 比重 | 1.065～1.070 g/cm³ |
| 粘度 | (15～20)×10⁴ mPa•s |
产品性能
- 低析出特性：本品析出量极低，可与盐溶液长期稳定共存，在生理盐水中（5g/100ml，23℃）放置48小时，PH值变化不超过0.5，可有效避免胶内物质析出污染PRP，保护生长因子活性，不干扰后续实验及临床应用。
- 无热源：整个生产过程在无菌条件下进行，严格控制原材料性能，确保产品无热源，符合医用级标准，保障PRP制备的安全性。
- 优异耐低温性能：16mm试管内的血样离心后，在-60℃冷冻48小时，分离胶层无变形、无析出，隔离效果保持完好。（对比说明：普通分离胶不耐低温冷冻，解冻后分离胶层易析出，隔离效果不完整；日本积水分离胶在低温冷冻后亦会出现明显凸起。）
- 无爬管现象：离心后血清（血浆）层管壁无分离胶残留，可避免堵针隐患，保障PRP取样顺畅，提升操作便捷性。
使用方法
本品主要用于PRP真空采血管的制备及PRP分离，具体操作如下：
1. 加胶操作：采用加胶机将本品加入试管中，加入量为0.8～1.0g/每支管（原则上以胶在试管中翻转厚度4mm-5mm为宜）。
2. 加胶辅助：本品最高限温为80℃，加胶时可采用水浴或其他合适方法加热，加热温度控制在60-70℃，加热时间以胶体温度基本均匀为宜，便于加胶操作。
3. 采血管制备：
- 加胶后应静置24小时以上方可离心，第一次以RCF 1200-1500g离心3-5分钟，排出胶体中残留气泡；
- 第二次以RCF=1800-2000g离心3～5分钟，使胶面平整；
- 离心后静置48小时，待分离胶结构稳定后，加入ACD或柠檬酸钠等抗凝剂，抽负压，24小时内进行辐照灭菌，完成PRP真空采血管制备（生产过程中可根据设备不同，调整相应工艺）。
4. PRP分离：采样后，在离心机上以RCF=2000～2500g离心8~10分钟，取分离胶上层1ml处的血浆，即为富含生长因子的富血小板血浆（PRP）。
5. 辐照与加热要求：建议辐照最大剂量不超过25kGy；如使用过程中需要加热，建议加热温度不超过60℃，短期温度不超过80℃。加热后分离胶会变黄，属于还原剂的正常反应，不影响产品性能及PRP制备效果。
注意事项
- 温度使用范围：本品推荐使用温度为-20～+80℃，粘度会随环境温度变化而波动。当环境温度低于15℃时，可能导致加胶困难，建议水浴加热至60～70℃，降低粘度，提高加胶效率。
- 加胶异常处理：常温加胶时若出现“拉丝”现象，可适当提高加胶温度，或通过振动方式将胶丝震断，确保加胶均匀。
- 胶体保护：本品经过高真空处理，胶体中基本无空气夹杂，转移、加胶过程中不建议搅拌，避免胶体中混入大量空气，影响隔离效果。
- 运输与储存注意：本品加入试管后，在真空状态下经60℃倒立试验20天以上，胶体平面流淌距离≤3mm，可保证集装箱方式60℃连续运输20天无异常。但仍建议长时间运输过程中，含分离胶的采血管不要倒置，防止激发胶的触变性，导致分离胶流淌。
- 加料斗清洗：更换不同厂家的分离胶，或加料斗长时间放置暴露在空气中、重新投入使用前，需彻底清理：先用不锈钢铲铲除加料斗四周表面的胶体，再用毛刷蘸DMC、D4等溶剂清洗，最后用抹布蘸溶剂擦拭干净；加料管也需同步清理，防止胶体交叉污染。
- 避光、防潮、密封保存，避免阳光直射及潮湿环境，防止胶体变质。
This product is a low-extraction PRP separation gel, specially used for the isolation and preparation of Platelet-Rich Plasma (PRP) rich in growth factors. It can effectively protect the activity of growth factors in plasma. This product can be combined with anticoagulants to prepare PRP vacuum blood collection tubes, which are widely used in clinical treatment, tissue repair and regeneration, aesthetic plastic surgery and other fields, providing efficient, clean and stable separation guarantee for PRP preparation.
| Main Ingredients | High-molecular polymer, silicon dioxide, etc. |
| Properties | This product is a yellow transparent colloid with good thixotropy and isolation effect. It is physiologically inert, does not react with blood components or anticoagulants, and does not interfere with the activity of growth factors in PRP. |
| Specific Gravity | 1.065～1.070 g/cm³ |
| Viscosity | (15～20)×10⁴ mPa•s |
Product Performance
- Low-extraction Property: This product has an extremely low extraction volume and can stably coexist with salt solutions for a long time. When placed in normal saline (5g/100ml, 23℃) for 48 hours, the pH change does not exceed 0.5. It can effectively avoid contamination of PRP by precipitated substances in the gel, protect the activity of growth factors, and does not interfere with subsequent experiments and clinical applications.
- Pyrogen-free: The whole production process is carried out under aseptic conditions, with strict control over raw material performance to ensure the product is pyrogen-free and meets medical-grade standards, guaranteeing the safety of PRP preparation.
- Excellent Low-temperature Resistance: After centrifugation of blood samples in 16mm test tubes and freezing at -60℃ for 48 hours, the separation gel layer shows no deformation or precipitation, and the isolation effect remains intact. (Comparative note: Ordinary separation gel is not resistant to low-temperature freezing; the gel layer tends to precipitate after thawing with incomplete isolation. Japan’s Sekisui separation gel also shows obvious bulging after low-temperature freezing.)
- No Tube Climbing: No separation gel residue is found on the tube wall of the serum (plasma) layer after centrifugation, avoiding the risk of needle blockage, ensuring smooth PRP sampling and improving operation convenience.
Instructions for Use
This product is mainly used for the preparation of PRP vacuum blood collection tubes and PRP separation. The specific operations are as follows:
- Gel Filling Operation: Use a gel filling machine to add this product into test tubes, with a dosage of 0.8～1.0g per tube (generally, the thickness of the inverted gel in the tube should be 4mm-5mm).
- Auxiliary Gel Filling: The maximum temperature limit of this product is 80℃. Water bath or other suitable heating methods can be used during gel filling, with the heating temperature controlled at 60-70℃ until the colloid temperature is basically uniform for easier operation.
- Blood Collection Tube Preparation:After gel filling, let it stand for more than 24 hours before centrifugation. First, centrifuge at RCF 1200-1500g for 3-5 minutes to remove residual bubbles in the colloid;Second, centrifuge at RCF 1800-2000g for 3-5 minutes to flatten the gel surface;After centrifugation, let it stand for 48 hours until the separation gel structure is stable. Then add anticoagulants such as ACD or sodium citrate, pump vacuum, and perform irradiation sterilization within 24 hours to complete the preparation of PRP vacuum blood collection tubes (the process can be adjusted according to different equipment).
- PRP Separation: After sampling, centrifuge at RCF 2000～2500g for 8~10 minutes. Take the plasma 1ml above the separation gel layer, which is Platelet-Rich Plasma (PRP) rich in growth factors.
- Irradiation and Heating Requirements: The recommended maximum irradiation dose is no more than 25kGy; if heating is required during use, the recommended temperature is no more than 60℃, and the short-term temperature is no more than 80℃. The colloid will turn yellow after heating, which is a normal reaction of the reducing agent and does not affect product performance or PRP preparation effect.
Precautions
- Temperature Range: The recommended operating temperature is -20～+80℃. Viscosity fluctuates with ambient temperature. Gel filling may be difficult when the ambient temperature is below 15℃; water bath heating to 60～70℃ is recommended to reduce viscosity and improve filling efficiency.
- Abnormal Gel Filling Treatment: If "stringing" occurs during room-temperature gel filling, properly increase the filling temperature or vibrate to break the gel strands to ensure uniform filling.
- Colloid Protection: This product has undergone high-vacuum treatment with almost no air inclusions. Stirring is not recommended during transfer and filling to avoid air mixing, which affects the isolation effect.
- Transportation and Storage: After being added to test tubes, the colloid has a flowing distance ≤3mm under vacuum after 60℃ inverted test for more than 20 days, ensuring no abnormality during 60℃ continuous container transportation for 20 days. However, it is recommended that blood collection tubes containing separation gel should not be inverted during long-term transportation to prevent triggering thixotropy and gel flowing.
- Hopper Cleaning: Before replacing separation gel from different manufacturers, or reusing hoppers exposed to air for a long time, thoroughly clean them: first scrape off colloid on the hopper surface with a stainless steel shovel, then clean with a brush dipped in solvents such as DMC and D4, and finally wipe with a cloth dipped in solvent; the feeding tube should also be cleaned simultaneously to prevent colloid cross-contamination.
- Store in a sealed, light-proof and moisture-proof place, avoid direct sunlight and humid environment to prevent colloid deterioration.
规格
别名
富血小板血浆分离胶 | PRP 专用低析出分离胶
英文别名
Platelet-Rich Plasma Separation Gel | PRP Separation Medium | PRP Gel Separator
规格或纯度
BioReagent,无菌
稳定性与储存
Store at room temperature long term (36 months). Store in the dark. Desiccated.
英文名称
PRP Low-Leaching Separation Gel
储存条件
避光,室温
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434400 |
分析证书 | L1520192 |
技术文档和文章
溶液计算器
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