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肝酯酶是脂肪分解酶，在肝实质细胞中合成，存在于肝脏窦周间隙内皮细胞表面和窦周间隙腔面的肝细胞微绒毛表面，可促进脂蛋白中的胆固醇向类固醇激素转化，血浆中的HL活性增高 时，血浆中小颗粒可导致LDL水平升高，加速动脉粥样硬化的发生。 测定原理：肝酯酶水解α-乙酸萘酯产生α-萘酚，可与固蓝B盐形成紫红色偶氮化合物，在595nm有特征吸收峰，颜色深浅在一定范围内与肝酯酶活性成正相关。
Hepatic lipase is a lipolytic enzyme synthesized in hepatocytes and localized on the surface of endothelial cells in the perisinusoidal spaces of the liver as well as on the microvilli of hepatocytes facing these spaces. It promotes the conversion of cholesterol in lipoproteins into steroid hormones. Elevated plasma HL activity can lead to increased levels of small LDL particles, thereby accelerating the development of atherosclerosis. Determination Principle: Hepatic lipase hydrolyzes α-naphthyl acetate to produce α-naphthol, which reacts with Fast Blue B salt to form a purple-red azo compound with a characteristic absorption peak at 595 nm. The color intensity is positively correlated with hepatic lipase activity within a certain range.
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