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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
L-半乳糖途径是合成AsA的主要途径。Gal LDH位于线粒体内膜,负责催化植物体内AsA生物合成的最后一步,也是该途径的关键酶之一,对植物体内AsA含量的积累起着至关重要的作用。 测定原理:Gal LDH催化L-半乳糖内酯还原细胞色素c(Cyt c),还原型Cyt c在550nm有吸收峰;测定还原型Cyt c增加速率,来计算Gal LDH活性。
The L-galactose pathway is the main route for AsA synthesis. Gal LDH is located on the inner mitochondrial membrane and catalyzes the final step of AsA biosynthesis in plants. It is also one of the key enzymes in this pathway and plays a crucial role in the accumulation of AsA content in plants. Assay principle: Gal LDH catalyzes the reduction of cytochrome c (Cyt c) by L-galactono-1,4-lactone. The reduced Cyt c has an absorption peak at 550 nm. Gal LDH activity is calculated by measuring the rate of increase in reduced Cyt c.