BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁生产的LumiDye™ 马来酰亚胺活化血蓝蛋白(SMCC-mcKLH)，即SMCC活化mcKLH，是由4-(N-马来酰亚胺基甲基)环已烷-1-羟酸琥珀酰亚胺酯(Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate, SMCC)修饰血蓝蛋白(Keyhole Limpet Hemocyanin, mcKLH)形成，携带马来酰亚胺基团，用于含半胱氨酸多肽以及其他巯基化抗原的半抗原-载体偶联。
钥孔戚血蓝蛋白(mcKLH)是高质量形式的KLH，从海水养殖生长的帽贝中获得，其免疫原性与KLH相似，但作为用于生产抗半抗原抗体的载体蛋白，具有更高的稳定性和效率。每个mcKLH分子可偶联多个抗原分子，以此提升抗原免疫原性，提高免疫动物针对目标表位产生高滴度抗血清的概率，同时mcKLH具有高分子质量（4.5×10⁵至 1.3×10⁷道尔顿；350和 390 kDa 亚基聚集体），可引发比BSA或OVA更强的免疫应答。因此常作为载体蛋白，与半抗原、弱免疫原偶联，用于动物免疫制备特异性抗体。
本产品为mcKLH与SMCC的连接产物，用500摩尔马来酰亚胺/摩尔mcKLH进行活化后，经纯化去除过量的SMCC，溶于含3%海藻糖的PBS-EDTA中，SMCC-mcKLH最终以冻干粉的形式提供，长期储存也不损失巯基反应性，保证了SMCC-mcKLH与其它含巯基分子等半抗原偶联的最佳性质。
使用方法
本产品最佳使用浓度为10mg/mL，加入200 uL纯水，摇晃瓶体使SMCC-mcKLH完全溶解后可进行标记。
注意：本产品需现用现配。SMCC-mcKLH形成一种呈半透明至蓝灰色的悬浮液。请勿涡旋振荡或加热该悬浮液，否则会导致载体蛋白沉淀。
图1 LumiDye™ 马来酰亚胺活化血蓝蛋白 (SMCC-mcKLH) (L1524015)与半抗原的偶联原理图
保存条件
4ºC避光保存，12个月内有效，切勿-20ºC或更低温度保存。
注意事项
1. 避免在反应过程中使用含有伯胺和巯基的缓冲液，以减少不必要的竞争反应。
2. 与之偶联的半抗原需含游离巯基。
3. 本产品的偶联产物，须避光保存，请勿冷冻。
4. 操作过程中，务必穿戴实验服、一次性手套及防护装备。
5. 所有产品仅限于研究用途。
LumiDye™ SMCC Activated Keyhole Limpet Hemocyanin (SMCC-mcKLH) is formed by modifying mcKLH with Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC). It contains a maleimide group that reacts with thiol groups of target molecules, such as haptens.
mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture. It exhibits immunogenicity comparable to KLH, yet provides enhanced stability and efficiency as a carrier protein for generating anti-hapten antibodies. Several antigen molecules can be conjugated to each mcKLH molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Meanwhile, KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390 kDa subunits) and elicits a stronger immune response than BSA or OVA. Consequently, mcKLH is a popular carrier protein for conjugation to haptens and other weak antigens to make them more immunogenic for antibody production.
This product is a purified conjugate of mcKLH and SMCC. It is activated with 500 moles of maleimide per mole of mcKLH, then purified to remove excess SMCC. Formulated in PBS-EDTA containing 3% trehalose, SMCC-mcKLH is ultimately supplied as a lyophilized powder. The long-term storage of this product does not affect its thiol reactivity, ensuring the optimal properties for conjugation with other thiol-containing molecules such as haptens.
Instruction for use
The optimal concentration for this product is 10 mg/mL. Add 200 μL of ultrapure water, gently swirl the bottle to ensure complete dissolution of the protein, then proceed with labeling.
Note: The SMCC-mcKLH solution must be prepared fresh before each use. SMCC-mcKLH forms a suspension that typically appears translucent to whitish-blue. Do not vortex or heat the suspension, which will precipitate the carrier.
Figure 1. Schematic diagram of the conjugation of LumiDye™ SMCC Activated Keyhole Limpet Hemocyanin (SMCC-mcKLH) (L1524015) to haptens.
Storage
Store at 4℃ in the dark for up to 12 months. Do not store at -20℃ or lower temperatures.
Matters needing attention
1. Avoid using buffers containing primary amines and thiols during the reaction to minimize unnecessary competitive reactions.
2. The hapten to be conjugated must contain free sulfhydryl groups.
3. Products conjugated with the SMCC-mcKLH should be stored at 4℃ in the dark. Do not freeze.
4. During operation, always wear a lab coat, disposable gloves, and protective equipment.
5. All products are for research use only.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0938850 |
分析证书 | L1524015 |
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