计算溶液所需的质量、体积或浓度。
Lipo3000转染试剂
有货
为什么选择此级别
BioReagent,无菌,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
是否无菌：是
Lipo3000转染试剂采用了先进的脂质纳米颗粒技术，实现绝佳转染性能和可重复性的结果。其可针对最广泛类型的常见及难转染细胞，实现超高转染效率，同时提供更高的细胞活力。Lipo3000转染试剂性质温和毒性低优化了转染过程的全部四个步骤，并结合先进的脂质纳米颗粒技术。绝佳的转染性能可以降低所需的试剂量，并降低所有可能对您的细胞系产生毒性的风险。对多种类型的细胞和培养板都具有高转染效率；转染时血清的存在不影响转染效率的优点。
适用范围：贴壁细胞和悬浮细胞（哺乳动物细胞系）的转染。
产品特性
1. 卓越的转染效率—针对最广泛类型的难转染细胞，可将效率提升3～10倍。
2. 作用温和，细胞毒性低—可改善细胞活力。
3. 高性价比高，同时实现顶级的转染结果。
产品组分及储存条件：
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使用方法 (DNA的转染)：
注：每次转染一定要进行预实验，来摸索最佳条件，尤其是转染试剂的最佳用量！
质粒DNA的转染初次使用，建议进行剂量优化实验（不同DNA:试剂比例），来摸索最佳条件，尤其是转染试剂的最佳用量!
对大多数细胞来说，DNA (μg) 与Lipo3000转染试剂 (μl) 的比例为1:2~1:3。转染时高的细胞密度可以得到高的转染效率和表达水平，并能减少细胞毒性。
质粒要求：小于8000bp，超螺旋构型，A₂₆₀/A₂₈₀=1.8–2.0，内毒素<0.1EU/μg.
使用方法 (siRNA转染)：
转染siRNA至细胞中时，完全遵循如上所述的DNA实验方案。
对大多数细胞来说，转染时高的细胞密度可以得到高的转染效率和表达水平，并能减少细胞毒性。
1.接种细胞至70-90%汇合度时转染。
2.使用Opti-MEM培养基稀释B液 (建议用2管，做两个预实验)，充分混匀。
3. 使用Opti-MEM培养基稀释DNA，制备DNA预混液，然后添加A液，充分混匀。
4. 在每管已稀释的B液中加入以上稀释的DNA (1:1比例)。
5. 室温孵育5～15分钟。
6. 加入DNA-脂质体复合物至细胞中。
7. 37°C孵育细胞2 -4天。然后分析转染细胞。
常见细胞的转染效率：（仅供参考，实验条件不同转染效率会有较大差别）
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细胞转染注意事项：
细胞/DNA/siRNA每次都不同，建议每次转染一定要进行预实验来摸索最佳条件，尤其是转染试剂的最佳用量！
1. 转染试验失败原因：DNA/siRNA，转染试剂和细胞。转染效率低要先排除一下是否是siRNA的问题，如果siRNA无效，换再好的转染试剂也没意义，可以用带荧光标记的control siRNA做一下看看，转进去的话会有荧光的。确实转染效率低，可以考虑换转染试剂；毒性大则要减少转染试剂的用量。
2. 细胞的种类和状态影响较大：转染时细胞必须处于良好生长状态，转染时细胞的密度一般铺板率在达到70—80%最好（此时细胞处于对数生长期）。
3. 如果是贴壁细胞，应保证贴壁在12～24小时在进行转染，否则细胞转染容易脱壁。对于贴壁生长细胞，一般要求在转染前一日，必须应用胰酶处理成单细胞悬液，重新接种于培养皿或瓶，转染当日的细胞密度以70-90%（贴壁细胞）或2×10⁶-4×10⁶细胞/ml（悬浮细胞）为宜，最好在转染前4h换一次新鲜培养液。
4. 质粒的大小，质量和用量对转染效率很关键。质粒要求：小于8000bp，超螺旋构型，A₂₆₀/A₂₈₀=1.8–2.0，内毒素<0.1EU/μg。
5. 转染时注意脂质体和用量，过量的话对细胞毒性大也容易失败。
6. 转染作用4～6小时一定记得要换含血清的培养基，以降低毒性。
7. 培养基以及洗涤细胞和稀释用的培养基要无血清、无双抗。
8. 转染试剂对个别细胞可能有一定毒性，在转染过程由于提高细胞的通透性因而不能在培养答基中添加抗生素。
9. 高纯度的DNA或RNA可获得较高的转染效率！用于转染的质粒DNA必须无蛋白质，无RNA和其他化学物质的污染，A₂₆₀/A₂₈₀应在1.8以上。血清中含有大量的蛋白质，在转染过程中，带负电的蛋白质可能干扰阳离子脂质体对核酸的吸附，影响转染效率。另外，使用脂质体等转染试剂时，由于含血清转染会将血清中的蛋白带入细胞，引发细胞毒性，导致转染效率降低，故用无血清培养基转染效果更好！
10. 培养基中的血清：在开始准备DNA和转染试剂稀释液时要使用无血清的培养基，因为血清会影响复合物的形成。其实，只要在DNA-转染试剂复合物形成时不含血清，在转染过程中是可以使用血清的。
11. 培养基中的抗生素：抗生素是影响转染的培养基添加物。这些抗生素一般对于真核细胞无毒，但阳离子脂质体试剂增加了细胞的通透性，使抗生素可以进入细胞。这降低了细胞的活性，导致转染效率降低。
12. 一般在转染24-48h，靶基因即在细胞内表达。根据不同的实验目的，24-48h后即可进行靶基因表达的检测实验。
13. 如若建立稳定的细胞系，则可对靶细胞进行筛选，根据不同基因载体中所含有的抗性标志选用相应的药物，常用的真核表达基因载体的标志物有潮霉素和新霉素等。
14. 建议设置阳性对照和阴性对照。
15. 不推荐用于：原代细胞（例如：Primary Hepatocytes）、神经元细胞（例如：Primary Neurons）、干细胞（例如：iPSCs）或高度敏感细胞（例如：MSCs，Tcells，Dendritic Cells）。
Lipo转染细胞死亡率高原因排查：
1. 细胞状态问题（最基础和关键的环节）
① 细胞状态不佳：转染前细胞本身就不健康（如传代次数过多、生长缓慢、形态异常），转染过程会加剧其死亡。确保转染时细胞处于对数生长期，活力旺盛（>90%）。
② 细胞密度不当：
⁕ 密度过低：细胞间没有足够的接触和支持，更容易受到转染试剂的毒性影响。
⁕ 密度过高：细胞进入接触抑制状态，分裂变慢，转染效率会显著下降，且容易导致毒性积累。请严格按照您使用的转染试剂和细胞类型的推荐密度进行铺板。
③ 支原体污染：支原体污染会严重改变细胞的代谢和生理状态，使其在转染压力下极易死亡。定期对细胞进行支原体检测。
2. 转染试剂与复合物毒性
① 转染试剂本身毒性：不同转染试剂（特别是阳离子聚合物和脂质体）有不同程度的细胞毒性。
② 复合物比例不当：
⁕ DNA/RNA与转染试剂比例不当：比例过高会导致形成过大、带正电过多的复合物，这些复合物会破坏细胞膜，导致死亡。
⁕ 复合物总量过多：加入的复合物总体积或总量过大，超过了细胞的承受能力。
③ 复合物孵育时间过长：将复合物与细胞共孵育的时间太长（如超过24小时），会持续对细胞产生毒性。通常6-8小时后更换新鲜培养基可以极大地缓解毒性。
④ 复合物制备不当：未使用无血清培养基稀释试剂和DNA，或混合顺序、涡旋力度不当，导致形成过大或不稳定的复合物。
3. 外源DNA/RNA的质量与数量
① DNA质粒质量差：制备的质粒含有内毒素、蛋白质、RNA或盐分残留，是导致细胞死亡的常见元凶。使用去内毒素的高纯度质粒提取试剂盒。
② DNA/RNA用量过高：过量的外源核酸会加重细胞的代谢负担，并可能引发强烈的天然免疫反应（尤其是对于RNA转染）。进行剂量梯度实验，找到最佳用量。
③基因产物本身的毒性：您要表达的蛋白本身可能对细胞有毒性（如促凋亡蛋白、离子通道蛋白、某些酶等），导致表达该蛋白的细胞死亡。
4. 转染操作过程
① 换液操作过于剧烈：在转染前换液或转染后换液时，吹打力度过大，直接将细胞吹落。
② 复合物加入方式：将复合物直接滴加到培养基中，而没有均匀滴洒，导致局部浓度过高。应缓慢、均匀地滴加到培养基的不同区域，并轻轻摇动培养皿使其混匀。
③使用了抗生素：在转染培养基中加入青霉素、链霉素等抗生素，会大大增加转染试剂的细胞毒性。转染全程必须使用无抗生素的培养基。
总结建议：
1. 检查细胞状态，确保健康且密度合适。
2. 设立Mock转染对照，这是判断毒性来源的“金标准”。
3. 确认质粒无内毒素，且转染过程中未使用抗生素。
4. 优化DNA/试剂比例并缩短孵育时间。
转染不成功原因：
通常由细胞状态、核酸质量、转染试剂选择不当或操作细节失误导致。以下是系统性原因分析与解决方案：
1. 细胞相关因素
① 细胞状态不佳：
处于对数生长期的细胞转染效率最高，静止期或老化细胞摄取能力下降。
建议：转染前确保细胞活力>90%，贴壁细胞汇合度控制在70%-90%，悬浮细胞密度为5×10⁵–2×10⁶个/mL。
② 细胞污染或传代次数过多：
支原体、细菌污染会显著降低转染效率，且难以察觉。
长期传代导致遗传变异，影响表达稳定性。
建议：定期检测支原体，使用低传代（<30次）细胞进行实验。
③ 细胞类型本身难转染：
原代细胞、神经元、干细胞等比HEK293、HeLa等常规细胞系更难转染。
解决方案：尝试电穿孔、病毒载体或专用转染试剂。
2. 核酸质量问题
① 纯度不足：蛋白质、DNA、有机溶剂残留会影响转染复合物形成。
检测方法：样品A₂₆₀/A₂₈₀应在1.8–2.0，A₂₆₀/A₂₃₀>2.0
② 核酸降解：RNA 易被RNase降解，需全程使用无酶耗材。
检测方法：琼脂糖凝胶电泳观察28S/18S条带是否清晰。
③ 内毒素污染(尤其质粒DNA): 内毒素可引发细胞毒性，导致死亡或转染失败。
建议：制备高纯度DNA。
3. 转染试剂与复合物制备
① 试剂选择不当：不同细胞类型需匹配不同试剂。
② 复合物比例与稳定性差：转染试剂与核酸比例需优化，过高毒性，过低效率低。
建议:按说明书配比，在无血清培养基中形成复合物，室温孵育15–20分钟。
③ 试剂毒性大：高浓度试剂引起细胞凋亡。
建议：通过CCK-8 或MTT法预实验确定最低有效剂量。
4. 操作与环境因素
① 转染时机不当：
转染后4–6小时应更换为完全培养基，去除残留试剂以减少毒性。
检测时间：DNA 转染后48–72 小时检测蛋白表达；siRNA 在24–72 小时检测沉默效果。
② 培养基含抗生素或血清干扰：脂质体转染时避免使用抗生素，可能增强毒性。复合物配制建议用无血清培养基 (如Opti-MEM)。
③ 无菌环境与物理参数：细菌/真菌污染会间接影响转染效率。温度、湿度波动影响细胞状态，应在稳定环境中操作。
Lipo3000 Transfection Reagent adopts advanced lipid nanoparticle technology to deliver exceptional transfection performance and reproducible results. It achieves ultra-high transfection efficiency across a broad range of common and hard-to-transfect cell types while maintaining superior cell viability. Lipo3000 Transfection Reagent is gentle and low in cytotoxicity. It optimizes all four steps of the transfection workflow with cutting-edge lipid nanoparticle technology. Its superior transfection performance reduces the required reagent dosage and minimizes potential toxic risks to your cell lines. It delivers high transfection efficiency across various cell types and culture plates, and its transfection efficiency remains unaffected by the presence of serum during transfection.
Application: Transfection of adherent and suspension mammalian cell lines.
Product Features
1. Exceptional transfection efficiency — improves transfection efficiency by 3 to 10 times for a wide variety of hard-to-transfect cells.
2. Gentle formulation with low cytotoxicity — helps preserve cell viability.
3. Cost-effective while achieving premium transfection results.
Product Components and Storage Conditions:
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Protocol (DNA Transfection):
Note: Always perform a pilot experiment to determine optimal conditions for each transfection, especially the optimal dosage of transfection reagent!
For the first-time plasmid DNA transfection, it is recommended to carry out a dose optimization study (varying DNA:reagent ratios) to identify the best conditions, particularly the optimal amount of transfection reagent.
For most cell types, the recommended ratio of DNA (μg) to Lipo3000 transfection reagent (μL) is 1:2 ~ 1:3. Higher cell density during transfection can improve transfection efficiency and gene expression levels, as well as reduce cytotoxicity.
Plasmid requirements: < 8000 bp, supercoiled conformation, A₂₆₀/A₂₈₀ = 1.8–2.0, endotoxin < 0.1 EU/μg.
Protocol (siRNA Transfection):
Follow the same general procedure described above for DNA transfection when delivering siRNA into cells.
For most cell types, higher cell density during transfection can improve transfection efficiency and gene silencing efficiency, as well as reduce cytotoxicity.
1. Seed cells and perform transfection when cells reach 70–90% confluency.
2. Dilute Solution B with Opti-MEM medium (prepare 2 replicate tubes for pilot experiments) and mix thoroughly.
3. Dilute DNA with Opti-MEM medium to prepare the DNA master mix, then add Solution A and mix thoroughly.
4. Add the diluted DNA mixture to each tube of diluted Solution B at a 1:1 volume ratio.
5. Incubate the mixture at room temperature for 5–15 minutes.
6. Add the DNA-liposome complex to the cells.
7. Incubate the cells at 37°C for 2–4 days, then analyze the transfected cells.
Transfection efficiency of common cells: (For reference only, transfection efficiency may vary greatly depending on experimental conditions)
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Notes on Cell Transfection:
Cells, DNA and siRNA vary between batches and experiments. It is recommended to perform pilot experiments every time to determine optimal conditions, especially the optimal dosage of transfection reagent!
1. Common causes of transfection failure include issues with DNA/siRNA, transfection reagent, and cell status. When experiencing low transfection efficiency, first verify the quality of the siRNA. If the siRNA itself is ineffective, changing transfection reagents will not improve results. A fluorescently labeled control siRNA can be used to check transfection success (fluorescence will be visible if uptake occurs). If true low transfection efficiency is confirmed, consider changing the transfection reagent; if excessive cytotoxicity is observed, reduce the dosage of transfection reagent.
2. Cell type and cell condition have a major impact on transfection results. Cells must be in good growth condition at the time of transfection; optimal cell confluency is generally 70–80% (cells in logarithmic growth phase).
3. For adherent cells, allow 12–24 hours for full attachment before transfection, otherwise cells may detach easily. For adherent cells, trypsinize into a single-cell suspension one day before transfection and re-seed in culture vessels. On the day of transfection, the optimal density is 70–90% confluency for adherent cells, or 2×10⁶–4×10⁶ cells/mL for suspension cells. It is recommended to replace with fresh culture medium 4 hours prior to transfection.
4. Plasmid size, quality and dosage are critical to transfection efficiency.
Plasmid requirements: < 8000 bp, supercoiled conformation, A₂₆₀/A₂₈₀ = 1.8–2.0, endotoxin < 0.1 EU/μg.
5. Pay attention to the dosage of liposomal transfection reagent. Excessive amounts cause severe cytotoxicity and transfection failure.
6. Replace with serum-containing culture medium 4–6 hours after transfection to reduce cytotoxicity.
7. Use serum-free and antibiotic-free medium for dilution, washing and transfection procedures.
8. Certain cell types may be sensitive to transfection reagents. Since transfection reagents increase cell membrane permeability, antibiotics should not be added to the culture medium during transfection.
9. High-purity DNA or RNA yields higher transfection efficiency. Plasmid DNA for transfection must be free of protein, residual RNA and other contaminants, with an A₂₆₀/A₂₈₀ ratio above 1.8. Serum contains abundant negatively charged proteins that can interfere with cationic liposome binding to nucleic acids and reduce transfection efficiency. Serum proteins may also enter cells during liposomal transfection and induce cytotoxicity. Therefore, serum-free medium is preferred for preparing transfection complexes.
10. Serum in culture medium: Use serum-free medium when preparing dilutions of DNA and transfection reagent, as serum interferes with complex formation. Serum can be present during incubation after the DNA-transfection reagent complexes have formed.
11. Antibiotics in culture medium: Antibiotics interfere with transfection. Although most antibiotics are non-toxic to intact eukaryotic cells, cationic liposomes increase membrane permeability and allow antibiotics to enter cells, reducing cell viability and transfection efficiency.
12. Target gene expression is typically detected 24–48 hours after transfection. Depending on experimental goals, gene expression analysis may be performed within this timeframe.
13. For establishment of stable cell lines, perform antibiotic selection using appropriate drugs corresponding to the resistance markers in the expression vectors. Common selection markers for eukaryotic vectors include hygromycin and neomycin (G418).
14. It is recommended to include positive and negative controls in all transfection experiments.
15. Not recommended for: primary cells (e.g., Primary Hepatocytes), primary neurons (e.g., Primary Neurons), stem cells (e.g., iPSCs), or highly sensitive cell types (e.g., MSCs, T cells, Dendritic Cells).
Investigation of High Cell Mortality Following Lipo Transfection:
1. Cell condition issues (the most fundamental and critical factor)
① Poor baseline cell health: Cells are already unhealthy prior to transfection (e.g., excessive passaging, slow growth, abnormal morphology), and the transfection process exacerbates cell death. Ensure cells are in the logarithmic growth phase with high viability (>90%) at the time of transfection.
② Improper cell seeding density:
⁕ Too low density: Cells lack sufficient cell-cell contact and support, making them more vulnerable to the cytotoxic effects of transfection reagents.
⁕ Too high density: Cells become contact-inhibited with slowed proliferation, resulting in markedly reduced transfection efficiency and cumulative cytotoxicity. Seed cells at the density recommended for your specific transfection reagent and cell line.
③ Mycoplasma contamination: Mycoplasma infection drastically alters cellular metabolism and physiology, rendering cells highly susceptible to death under transfection stress. Conduct regular mycoplasma testing.
2. Cytotoxicity of transfection reagents and nucleic acid complexes
① Intrinsic reagent cytotoxicity: Different transfection reagents (especially cationic polymers and liposomes) exhibit varying levels of inherent cytotoxicity.
② Incorrect complex formulation ratios:
⁕ Imbalanced DNA/RNA-to-reagent ratio: Excessively high ratios generate oversized, highly positively charged complexes that damage cell membranes and cause cell death.
⁕ Excessive total complex dosage: An overly large total volume or mass of complexes exceeds the cellular tolerance threshold.
③ Excessive complex incubation time: Extended incubation of complexes with cells (e.g., over 24 hours) causes sustained cytotoxicity. Replacing with fresh medium after 6–8 hours generally greatly reduces toxicity.
④ Improper complex preparation: Failure to dilute reagents and DNA in serum-free medium, incorrect mixing order, or excessive vortexing leads to the formation of oversized or unstable complexes.
3. Quality and dosage of exogenous DNA/RNA
① Poor plasmid DNA quality: Endotoxin, protein, residual RNA, or salt contaminants in plasmid preparations are a major cause of cell death. Use endotoxin-free high-purity plasmid purification kits.
② Excessive DNA/RNA dosage: Overloading cells with exogenous nucleic acids increases metabolic stress and may trigger robust innate immune responses (especially for RNA transfection). Perform dose-gradient experiments to determine the optimal dosage.
③ Toxicity of the target gene product: The expressed protein itself may be cytotoxic (e.g., pro-apoptotic proteins, ion channel proteins, certain enzymes), resulting in death of transfected cells.
4. Transfection operational procedures
① Harsh medium exchange: Vigorous pipetting during medium changes before or after transfection directly dislodges adherent cells.
② Uneven complex addition: Direct concentrated addition of complexes creates localized high concentrations. Add complexes slowly and evenly across the culture surface, then gently rock the plate for uniform distribution.
③ Antibiotic supplementation: The presence of antibiotics such as penicillin and streptomycin in transfection medium significantly increases lipofectamine cytotoxicity. Use antibiotic-free medium throughout the entire transfection process.
Summary Recommendations:
1. Check cell condition to ensure cells are healthy and at an appropriate density.
2. Include a mock transfection control, which serves as the gold standard for identifying sources of cytotoxicity.
3. Verify that plasmids are endotoxin-free and that no antibiotics are used during transfection.
4. Optimize the DNA/reagent ratio and reduce incubation time.
Causes of Transfection Failure:
Transfection failure is usually caused by poor cell condition, low-quality nucleic acids, inappropriate transfection reagent selection, or errors in experimental procedures. Below is a systematic analysis of potential causes and corresponding solutions:
1. Cell-Related Factors
① Poor Cell Condition
Cells in the logarithmic growth phase achieve the highest transfection efficiency, while quiescent or senescent cells exhibit reduced uptake capacity.
Recommendation: Ensure cell viability exceeds 90% before transfection. For adherent cells, maintain a confluency of 70%–90%; for suspension cells, adjust the density to 5×10⁵–2×10⁶ cells/mL.
② Microbial Contamination or Excessive Passaging
Mycoplasma and bacterial contamination markedly reduce transfection efficiency and are often difficult to detect.
Long-term repeated passaging leads to genetic variation and impairs transgene expression stability.
Recommendation: Perform regular mycoplasma testing, and use cells with low passage numbers (<30 passages) for experiments.
③ Intrinsically Hard-to-Transfect Cell Types
Primary cells, neurons, stem cells and other cell types are much harder to transfect than common cell lines such as HEK293 and HeLa.
Solutions: Try electroporation, viral vectors, or specialized transfection reagents.
2. Nucleic Acid Quality Issues
① Insufficient Purity
Residual proteins, genomic DNA, and organic solvents disrupt the formation of transfection complexes.
Quality check: Ensure the A₂₆₀/A₂₈₀ ratio is 1.8–2.0, and the A₂₆₀/A₂₃₀ ratio is greater than 2.0.
② Nucleic Acid Degradation
RNA is susceptible to RNase degradation; use RNase-free consumables throughout the entire process.
Quality check: Verify intact 28S/18S rRNA bands via agarose gel electrophoresis.
③ Endotoxin Contamination (especially for plasmid DNA)
Endotoxins induce cytotoxicity, resulting in cell death and transfection failure.
Recommendation: Prepare high-purity endotoxin-free plasmid DNA.
3. Transfection Reagents and Complex Preparation
① Inappropriate Reagent Selection
Different cell types require matched transfection reagents.
② Improper Ratio and Instability of Transfection Complexes
The ratio of transfection reagent to nucleic acid must be optimized: excessive reagent causes cytotoxicity, while insufficient reagent results in low transfection efficiency.
Recommendation: Follow the manufacturer’s instructions. Form complexes in serum-free medium and incubate at room temperature for 15–20 minutes.
③ Excessive Reagent Cytotoxicity
Over-concentrated transfection reagents trigger cell apoptosis.
Recommendation: Conduct preliminary CCK-8 or MTT assays to determine the minimum effective working concentration.
4. Operational and Environmental Factors
① Improper Timing of Medium Exchange and Detection
Replace with complete medium 4–6 hours after transfection to remove residual transfection reagent and reduce cytotoxicity.
Detection schedule: Assess protein expression 48–72 hours after DNA transfection; evaluate siRNA knockdown efficiency 24–72 hours after transfection.
② Interference from Antibiotics and Serum
Avoid antibiotics during liposome transfection, as they may amplify cytotoxicity. Prepare transfection complexes using serum-free medium (e.g., Opti-MEM).
③ Aseptic Conditions and Physical Parameters
Bacterial/fungal contamination indirectly impairs transfection efficiency. Fluctuations in temperature and humidity compromise cell health; perform experiments in a stable cell culture environment.
规格
别名
脂质体3000转染试剂
英文别名
Lipo3000 | Lipofectamine 3000
规格或纯度
BioReagent, 用于细胞培养, 无菌
稳定性与储存
Store at 2-8℃ long term (12 months). Do not freeze.
英文名称
Lipo3000 Transfection Reagent
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
产品线
生物产品
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0938968 |
分析证书 | L1525371 |
| ZJ26F0938967 |
分析证书 | L1525371 |
技术文档和文章
溶液计算器
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