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Leibovitz's L-15培养基的缓冲系统由磷酸盐和游离碱基氨基酸组成,代替了传统的碳酸氢钠缓冲系统,同时,用半乳糖和丙酮酸钠代替葡萄糖可以防止酸性代谢副产物的形成,有助于维持培养液PH的稳定,适合用于非CO2平衡环境的细胞培养。Leibovitz's L-15培养基适用于猴肾细胞和HEP-2的培养、原代(如胚胎和成人组织)细胞分离、多种病毒的培养以及神经元的培养等。 本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分;但不含蛋白质、脂类或任何生长因子;故此产品需搭配血清或无血清添加物使用。 注意事项 1、本产品经过滤除菌,使用时应注意无菌操作,避免污染; 2、为保持本产品的最佳使用效果,请勿进行冻融处理; 3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
Leibovitz's L-15 medium utilizes a buffering system composed of phosphates and free‑base amino acids, replacing the conventional sodium bicarbonate buffer system. In addition, it substitutes galactose and sodium pyruvate for glucose to prevent the formation of acidic metabolic by‑products, thereby helping to maintain a stable pH in the culture medium. This makes it suitable for cell culture under non‑CO₂‑equilibrated environments. Leibovitz's L‑15 medium is applicable for the culture of monkey kidney cells and HEP‑2 cells, isolation of primary cells (e.g., from embryonic and adult tissues), culture of various viruses, and neuronal culture, among others. This product contains multiple components required for cell culture, including amino acids, vitamins, inorganic salts, etc.; however, it contains no proteins, lipids, or any growth factors. Therefore, this product must be used with serum or serum‑free supplements. Precautions 1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. 2. To maintain optimal performance, avoid freeze‑thaw cycles. 3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.