本试剂盒利用L-乳酸脱氢酶（L-LDH）催化L-乳酸生成丙酮酸，同时将NAD⁺还原为NADH和H⁺。生成的NADH通过PMS的递氢作用，将氧化型噻唑蓝（MTT）还原为甲臜，该产物在570 nm处具有特征吸收峰。通过测定该波长下吸光度的变化，即可定量检测样品中L-乳酸的含量。
This kit utilizes L-lactate dehydrogenase (L-LDH) to catalyze the conversion of L-lactate to pyruvate, while simultaneously reducing NAD⁺ to NADH and H⁺. The generated NADH, through the electron-transferring action of phenazine methosulfate (PMS), reduces thiazolyl blue tetrazolium bromide (MTT) to formazan, which exhibits a characteristic absorbance peak at 570 nm. By measuring the change in absorbance at this wavelength, the L-lactate content in the sample can be quantitatively determined.
危险品化学品经营许可证（带存储）