计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒利用L-乳酸脱氢酶(L-LDH)催化L-乳酸生成丙酮酸,同时将NAD⁺还原为NADH和H⁺。生成的NADH通过PMS的递氢作用,将氧化型噻唑蓝(MTT)还原为甲臜,该产物在570 nm处具有特征吸收峰。通过测定该波长下吸光度的变化,即可定量检测样品中L-乳酸的含量。
This kit utilizes L-lactate dehydrogenase (L-LDH) to catalyze the conversion of L-lactate to pyruvate, while simultaneously reducing NAD⁺ to NADH and H⁺. The generated NADH, through the electron-transferring action of phenazine methosulfate (PMS), reduces thiazolyl blue tetrazolium bromide (MTT) to formazan, which exhibits a characteristic absorbance peak at 570 nm. By measuring the change in absorbance at this wavelength, the L-lactate content in the sample can be quantitatively determined.