BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用选择性表面活性剂法直接测定低密度脂蛋白胆固醇（LDL-C）。在胆固醇酯酶（CHER）和胆固醇氧化酶（CHOD）组成的测定体系中，加入特异性表面活性剂，可选择性地使LDL-C溶解并释放胆固醇，参与后续酶促反应；而HDL、VLDL及乳糜微粒等脂蛋白因受到表面活性剂与糖化合物的共同抑制，不参与反应，仍以完整脂蛋白形式残存于反应液中，从而实现LDL-C的直接测定。 其具体检测原理如下：LDL-C中释放的胆固醇酯在酯酶催化下水解生成游离胆固醇（FC）；FC在胆固醇氧化酶作用下被氧化生成4-胆甾烯酮和过氧化氢（H₂O₂）；在过氧化物酶（POD）催化下，H₂O₂与4-氨基安替比林（4-AAP）及酚类显色剂发生氧化偶联反应，生成红色醌亚胺化合物，该产物在546 nm处具有特征吸收峰。通过测定该波长下吸光值的增幅，结合标准曲线即可精准计算样品中LDL-C的含量。
This kit employs a selective surfactant method for the direct determination of low-density lipoprotein cholesterol (LDL-C). In the assay system composed of cholesterol esterase (CHER) and cholesterol oxidase (CHOD), a specific surfactant is added to selectively solubilize LDL-C and release cholesterol, which then participates in the subsequent enzymatic reactions. Meanwhile, other lipoproteins such as HDL, VLDL, and chylomicrons are inhibited by the combined action of the surfactant and sugar compounds, and thus do not participate in the reactions but remain as intact lipoproteins in the reaction solution, enabling the direct determination of LDL-C. The specific principle of the assay is as follows: The cholesterol esters released from LDL-C are hydrolyzed by cholesterol esterase to generate free cholesterol (FC). FC is then oxidized by cholesterol oxidase to produce 4-cholestenone and hydrogen peroxide (H₂O₂). In the presence of peroxidase (POD), H₂O₂ undergoes an oxidative coupling reaction with 4-aminoantipyrine (4-AAP) and a phenolic chromogenic reagent to form a red quinone imine compound, which exhibits a characteristic absorbance peak at 546 nm. By measuring the increase in absorbance at this wavelength and referencing a standard curve, the LDL-C content in the sample can be precisely calculated.
危险品化学品经营许可证（带存储）