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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
赖氨酸是人体必需氨基酸之一，能促进生长发育、增强免疫功能，并具有提高中枢神经组织功能的作用。由于谷物食品中赖氨酸含量甚低，且在加工过程中易被破坏而缺乏，因此被称为第一限制性氨基酸。 本试剂盒的测定原理为：蛋白质中的赖氨酸含有一个游离的ε-NH₂，其与茚三酮试剂反应生成蓝紫色物质，且颜色的深浅在一定范围内与赖氨酸含量呈线性关系。因亮氨酸与赖氨酸碳原子数相同，且仅含一个游离氨基（ε-NH₂），故通常使用亮氨酸配制标准溶液。通过测定吸光度，即可定量计算样品中赖氨酸含量。
Lysine is one of the essential amino acids for humans. It promotes growth and development, enhances immune function, and plays a role in improving the function of the central nervous system. Because the lysine content in cereal foods is very low and is readily destroyed during processing, it is often referred to as the first limiting amino acid. The assay principle of this kit is as follows: lysine in proteins contains a free ε‑NH₂ group, which reacts with ninhydrin reagent to form a blue‑purple colored product. The color intensity is linearly related to the lysine content within a certain range. Since leucine has the same number of carbon atoms as lysine and contains only one free amino group (ε‑NH₂), leucine is commonly used to prepare the standard solution. The lysine content in the sample can be quantitatively calculated by measuring the absorbance.
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