BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用LDH-GPT偶联比色法快速测定乳酸含量。乳酸经乳酸脱氢酶催化生成丙酮酸和NADH；添加的谷氨酸-丙酮酸转氨酶分解丙酮酸，拉动反应平衡，确保NADH持续生成。NADH与显色剂反应生成450 nm处有特征吸收的有色产物，其吸光度增幅与乳酸含量成正比，据此实现定量检测。乳酸水平异常与糖尿病、酸中毒等疾病密切相关，本方法可为临床诊断提供参考依据。
This kit employs an LDH‑GPT coupled colorimetric method for the rapid determination of lactate content. Lactate is catalyzed by lactate dehydrogenase to generate pyruvate and NADH; the added glutamate‑pyruvate transaminase (GPT) decomposes pyruvate, driving the reaction equilibrium forward and ensuring continuous NADH production. The NADH then reacts with a chromogenic reagent to form a colored product with a characteristic absorption peak at 450 nm. The increase in absorbance is proportional to the lactate content, enabling quantitative detection. Abnormal lactate levels are closely associated with diseases such as diabetes mellitus and acidosis; this method can provide a reference basis for clinical diagnosis.
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