计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脂肪酶(LPS),又称甘油酯水解酶,催化甘油三酯水解生成脂肪酸和甘油(或甘油二酯及单酯),广泛分布于各类生物体中。血清中LPS水平异常升高常见于胰腺炎和胰腺癌,是临床胰腺疾病诊断的重要辅助指标之一。本试剂盒采用铜皂法测定LPS活性:LPS催化油脂水解生成游离脂肪酸;游离脂肪酸与铜离子反应生成有色铜皂复合物,该产物在710 nm(常用波长,具体以实际检测波长为准)处具有特征吸收峰。通过测定该波长下吸光度的增加速率,反映脂肪酸的生成速率,即可定量计算LPS的酶活性。
Lipase (LPS), also known as glyceride hydrolase, catalyzes the hydrolysis of triglycerides to fatty acids and glycerol (or diglycerides and monoglycerides), and is widely distributed in various organisms. Abnormal elevation of serum LPS levels is commonly observed in pancreatitis and pancreatic cancer, making it an important auxiliary indicator for the clinical diagnosis of pancreatic diseases. This kit employs the copper soap method for the determination of LPS activity: LPS catalyzes the hydrolysis of oils to release free fatty acids. The free fatty acids then react with copper ions to form a colored copper soap complex, which exhibits a characteristic absorption peak at 710 nm (commonly used wavelength; refer to the actual detection wavelength specified in the product manual). By measuring the rate of increase in absorbance at this wavelength, which reflects the rate of fatty acid production, the LPS enzyme activity can be quantitatively calculated.