计算溶液所需的质量、体积或浓度。
BioReagent,即用型,适用于免疫荧光(IF),适用于荧光分析 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
别藻蓝蛋白(APC)是提取自螺旋藻(Spirulina sp.)的藻胆蛋白,凭借高摩尔吸光系数与优异量子产率,被广泛应用于流式细胞术、免疫分析等生物检测场景。但天然别藻蓝蛋白存在固有缺陷:蛋白在低浓度环境下易发生亚基解离,制约了其在高灵敏度检测体系中的实际使用效果。
本产品为化学交联型别藻蓝蛋白(CL‑APC),通过共价交联方式稳定结合 α、β 亚基。在完整保留天然 APC 优良光谱学特征的基础上,大幅提升蛋白分子结构稳定性,从根本上改善低浓度条件下的解离难题。
产品形态
即用型液体,于专用保护缓冲体系中配制。无需脱盐,开盖即用。
核心产品优势
• 稳定性强:经化学交联修饰,可适配低浓度工作环境,实验重复性更好;
• 使用便捷:预优化保护缓冲液体系,无需脱盐处理,直接稀释即可开展实验;
• 荧光性能优良:维持 APC 固有荧光光谱(激发波长 / 发射波长:650 nm / 660 nm),适配流式细胞术、ELISA、生物微阵列等多种检测平台。
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Allophycocyanin (APC) is a phycobiliprotein extracted from Spirulina sp.. Boasting a high molar extinction coefficient and excellent quantum yield, it is widely applied in bio‑detection scenarios such as flow cytometry and immunoassays. Nevertheless, native allophycocyanin has inherent drawbacks: its subunits tend to dissociate under low‑concentration conditions, which limits its practical performance in high‑sensitivity detection systems.
This product is chemically cross‑linked allophycocyanin (CL‑APC). Its α and β subunits are stably bound via covalent cross‑linking. While fully preserving the favorable spectroscopic properties of native APC, it features greatly enhanced molecular structural stability, fundamentally resolving the subunit dissociation issue at low concentrations.
Product Form
Ready‑to‑use liquid, formulated in a dedicated protective buffer. No desalting required; use directly upon opening.
Key Product Advantages
• High stability: Chemically cross‑linked modification enables compatibility with low‑concentration working conditions and delivers improved experimental reproducibility.
• Convenient handling: Pre‑optimized protective buffer; experiments can be performed by direct dilution without desalting.
• Superior fluorescence performance: Retains the intrinsic fluorescence spectrum of APC (Excitation / Emission: 650 nm / 660 nm), compatible with multiple detection platforms including flow cytometry, ELISA and bio‑microarrays.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1529060 | |
| 分析证书 | L1529060 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Suitable for Immunofluorescence(IF) 级别指南 → 查看 BioReagent 级别指南 → 查看 Ready-to-use 级别指南 → 查看 for Fluorescence analysis 级别指南 →