计算溶液所需的质量、体积或浓度。
革兰氏碘液 (0.34%)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
革兰氏碘液为碘与碘化钾的水溶液，由 Lugol 于 1829 年发明并以其姓氏命名。该试剂可用作氧化型消毒剂、杀菌剂，用于饮用水应急消毒；实验室场景下可用于淀粉检测。
本品采用经典配方，碘含量 0.34%，主要应用包含：检测叶片、食品等样品中的淀粉；细胞染色，使细胞核着色便于观察；阴道镜 Schiller 试验，用于阴道及宫颈病变筛查 —— 正常组织因富含糖原被染为棕褐色，癌变组织不着色而呈浅色；同时可作为氧化型杀菌剂。在微生物实验中，革兰氏碘液常作为媒染剂，与结晶紫、沙黄配套，用于革兰氏细菌染色。
仅供科研使用，不可用于临床诊断及其它用途。
操作步骤 (仅供参考):
1、一般革兰氏细菌的涂片染色 1~2 min，组织切片应适当延长染色时间，特殊实验应根据要求操作。
注意事项:
1、涂片之前应事先在背面做好圆圈标记，以便判断后续试验的位置。
2、革兰氏染色的关键在于严格掌握脱色程度，脱色时间应根据经验判断。脱色过度，阳性菌可被误染为阴性菌；脱色不够，阴性菌可被误染为阳性菌。脱色可采用 70% 的乙醇。
3、阳性菌培养时间过长或已死亡或细菌溶解，都常呈阴性反应。
Gram’s Iodine Solution is an aqueous solution of iodine and potassium iodide, invented by Lugol in 1829 and named after him. It can be used as an oxidative disinfectant and bactericide for emergency disinfection of drinking water, as well as for starch detection in laboratory settings.
This product adopts a classic formulation with an iodine content of 0.34%. Its main applications include: detecting starch in samples such as leaves and food; cell staining to stain cell nuclei for easier observation; the Schiller test in colposcopy for screening vaginal and cervical lesions — normal tissues stain brown due to high glycogen content, whereas cancerous tissues remain unstained and appear pale; it also acts as an oxidative bactericide. In microbiological experiments, Gram’s Iodine Solution is commonly used as a mordant, combined with crystal violet and safranin for Gram bacterial staining.
For research use only. Not for clinical diagnosis or other applications.
Procedure (For reference only):
1. Stain general Gram‑stain bacterial smears for 1‑2 min. Appropriately prolong the staining time for tissue sections. Operate according to specific requirements for special experiments.
Precautions:
1. Draw circular marks on the reverse side of slides before smearing to locate the target area in subsequent experiments.
2. Proper decolorization is critical for Gram staining, and decolorization time should be determined empirically. Excessive decolorization may result in false‑negative results for Gram‑positive bacteria; insufficient decolorization may produce false‑positive results for Gram‑negative bacteria. 70% ethanol can be used for decolorization.
3. Gram‑positive bacteria may yield false‑negative results if cultured for too long, when dead, or after bacterial lysis.
规格
规格或纯度
BioReagent, 生物染色剂, 用于显微镜, 0.34%
稳定性与储存
Store at Room temperature long term (24 months). Store in the Dark.
英文名称
Lugol Solution（0.34%，For Gram Stain）
储存条件
室温
运输条件
常规运输
产品线
生物产品
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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